Wang Yunxia, Yang Sheng-Tao, Wang Yanli, Liu Yuanfang, Wang Haifang
Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai 200444, China.
Colloids Surf B Biointerfaces. 2012 Sep 1;97:62-9. doi: 10.1016/j.colsurfb.2012.04.013. Epub 2012 Apr 13.
Carbon nanotubes (CNTs) show promise as nano-drug carriers. To develop the CNT-based drug delivery systems, drug loading and release are two major issues. In this study, we systematically evaluated the adsorption and desorption of doxorubicin (DOX) on oxidized multi-walled CNTs (O-MWCNTs). Our results indicated that O-MWCNTs possessed a huge adsorption capacity for DOX (9.45×10(3) mg/g). Although the adsorption process was quite slow, the adsorption capacity kept high enough for the therapy while shortening the incubation time to 2h (1.03×10(3) mg/g). The desorption of DOX from O-MWCNTs scarcely occurred while incubated in buffer solution at both pH 7.4 and pH 5.5, however, the lower pH did benefit the desorption. The presence of serum proteins facilitated the desorption of DOX significantly, because these proteins bound strongly to O-MWCNTs resulting in the partial surface of O-MWNCTs being occupied. Moreover, the adsorption time also affected the release of DOX from O-MWCNTs. Shortening the incubation time benefited the release of DOX. The implications to the drug loading and therapeutics of the CNT-based drug delivery systems are discussed.
碳纳米管（CNTs）有望成为纳米药物载体。为了开发基于碳纳米管的药物递送系统，药物负载和释放是两个主要问题。在本研究中，我们系统地评估了阿霉素（DOX）在氧化多壁碳纳米管（O-MWCNTs）上的吸附和解吸情况。我们的结果表明，O-MWCNTs对DOX具有巨大的吸附容量（9.45×10(3) mg/g）。尽管吸附过程相当缓慢，但在将孵育时间缩短至2小时（1.03×10(3) mg/g）的情况下，吸附容量仍保持足够高以用于治疗。当在pH 7.4和pH 5.5的缓冲溶液中孵育时，DOX从O-MWCNTs上几乎不发生解吸，然而，较低的pH确实有利于解吸。血清蛋白的存在显著促进了DOX的解吸，因为这些蛋白与O-MWCNTs强烈结合，导致O-MWNCTs的部分表面被占据。此外，吸附时间也影响了DOX从O-MWCNTs上的释放。缩短孵育时间有利于DOX的释放。本文讨论了这些结果对基于碳纳米管的药物递送系统的药物负载和治疗的意义。
