Yang Tao, Wu Zhenzhen, Wang Pingting, Mu Tingting, Qin Han, Zhu Zhimin, Wang Jian, Sui Lei
Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, China No.14, 3rd Section of Ren Min Nan Rd., Chengdu, Sichuan, 610041, China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China No.14, 3rd Section of Ren Min Nan Rd., Chengdu, Sichuan, 610041, China.
J Mater Sci Mater Med. 2017 Jul;28(7):110. doi: 10.1007/s10856-017-5920-9. Epub 2017 Jun 6.
A novel dual-drug delivery system (DDDS) for cancer chemotherapy has been established by employing highly purified and mildly oxidized large-inner-diameter multi-walled carbon nanotubes (LID-MWCNTs) as the vector. The LID-MWCNTs were modified with the antitumor drugs, cisplatin (CDDP) and doxorubicin (DOX). CDDP was encapsulated inside the nanotube vectors by a wet-chemical approach while DOX was attached to the external surfaces through non-covalently interaction. The loading efficiencies of CDDP and DOX were as high as 84.56 and 192.67%, respectively. Notably, after CDDP was encapsulated inside the nanotubes, a three-level blocking strategy, which included polyethylene glycol, folic acid and DOX, was employed to block the CDDP exits at different levels. The pH-sensitive release profile of CDDP was demonstrated using a modified characterization method, as well as that of DOX. Finally, the anticancer activity of the DDDS on MCF-7 cells was tested and a synergistic effect was recorded. This work is part of our LID-MWCNTs based drug delivery system studies, and provides a basis for developing a novel comprehensive antitumor treatment that combines chemotherapy and photothermal therapy.
通过使用高度纯化且轻度氧化的大内径多壁碳纳米管（LID-MWCNTs）作为载体，建立了一种用于癌症化疗的新型双药递送系统（DDDS）。LID-MWCNTs用抗肿瘤药物顺铂（CDDP）和阿霉素（DOX）进行了修饰。CDDP通过湿化学方法封装在纳米管载体内，而DOX通过非共价相互作用附着在其外表面。CDDP和DOX的负载效率分别高达84.56%和192.67%。值得注意的是，在CDDP封装在纳米管内之后，采用了一种三级阻断策略，包括聚乙二醇、叶酸和DOX，以在不同水平阻断CDDP的流出。使用改进的表征方法证明了CDDP以及DOX的pH敏感释放特性。最后，测试了DDDS对MCF-7细胞的抗癌活性，并记录到了协同效应。这项工作是我们基于LID-MWCNTs的药物递送系统研究的一部分，为开发一种结合化疗和光热疗法的新型综合抗肿瘤治疗方法提供了基础。
