Shundo Chigusa, Zhang Hong, Nakanishi Takuya, Osaka Tetsuya
Department of Applied Chemistry, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Colloids Surf B Biointerfaces. 2012 Sep 1;97:221-5. doi: 10.1016/j.colsurfb.2012.04.003. Epub 2012 Apr 13.
Magnetite nanoparticles are expected to be applied in the medical field because of their biocompatibility and high saturated magnetization. In this paper, magnetite nanoparticles with a diameter of approximately 40 nm were evaluated for their safety by using mouse embryonic stem (mES) cells. First, various doses of magnetite nanoparticles were added to mES cells to find an optimal dose and to evaluate viability and keeping undifferentiated states of mES. The uptake of nanoparticles by mES cells was confirmed by using cytospin and transmission electron microscopy. Next, mES cells containing magnetite nanoparticles were collected by a magnet column 24h after the addition of magnetite nanoparticles, and the change in the ratio of those mES cells to the total mES cells was assayed by FACS 0, 4, 8, 12, 16, 24, 48 and 72 h after incubation. The result showed that the ratio decreased with time, indicating that the mES cells excreted the nanoparticles, for there was no change in the total number of cells. Based on these results, it was concluded that magnetite nanoparticles were safe to mES cells.
由于其生物相容性和高饱和磁化强度，磁铁矿纳米颗粒有望应用于医学领域。在本文中，使用小鼠胚胎干细胞（mES）对直径约为40 nm的磁铁矿纳米颗粒的安全性进行了评估。首先，将不同剂量的磁铁矿纳米颗粒添加到mES细胞中，以找到最佳剂量，并评估mES细胞的活力和维持未分化状态的能力。通过细胞涂片和透射电子显微镜确认了mES细胞对纳米颗粒的摄取。接下来，在添加磁铁矿纳米颗粒24小时后，用磁柱收集含有磁铁矿纳米颗粒的mES细胞，并在孵育0、4、8、12、16、24、48和72小时后，通过流式细胞术分析这些mES细胞与总mES细胞的比例变化。结果表明，该比例随时间下降，这表明mES细胞排出了纳米颗粒，因为细胞总数没有变化。基于这些结果，得出结论：磁铁矿纳米颗粒对mES细胞是安全的。
