State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Bioresour Technol. 2012 Jul;116:74-9. doi: 10.1016/j.biortech.2012.03.100. Epub 2012 Apr 13.
A modified method for calculating practical ethanol yield in the simultaneous saccharification and fermentation (SSF) at high lignocellulosic solids content and high ethanol titer is proposed considering the liquid volume change caused by high titer ethanol generation and the water consumed during cellulose degradation. This modified method was applied to determine the practical ethanol yields of several practical SSF operations and the results compared to those using the conventional method. The results show that the liquid volume increase with ethanol formation during SSF was approximately five times greater than the volume decrease duo to water consumption during cellulose degradation. Furthermore, the practical ethanol yields calculating using traditional method were underestimated and the underestimated errors increased with the increasing ethanol titer. The present work may provide a convenient and accurate method for calculating practical ethanol yield in a high solids and high ethanol titer SSF systems.
提出了一种改进的方法，用于计算高木质纤维素固体含量和高乙醇浓度下同步糖化发酵（SSF）中的实际乙醇产率，该方法考虑了高乙醇浓度下产生的液体体积变化以及纤维素降解过程中消耗的水。将该改进方法应用于几种实际 SSF 操作的实际乙醇产率的确定，并将结果与传统方法进行比较。结果表明，在 SSF 过程中，乙醇形成导致的液体体积增加约为纤维素降解过程中消耗的水量减少的五倍。此外，使用传统方法计算的实际乙醇产率被低估，并且低估误差随着乙醇浓度的增加而增加。本研究可为高固体和高乙醇浓度 SSF 系统中实际乙醇产率的计算提供一种简便、准确的方法。
