State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang, Henan 473000, China.
Bioresour Technol. 2014 Apr;157:6-13. doi: 10.1016/j.biortech.2014.01.060. Epub 2014 Feb 1.
Industrial waste corncob residues (CCR) are rich in cellulose and can be hydrolyzed directly without pretreatment. However, a poor fermentation performance was frequently observed in the simultaneous saccharification and ethanol fermentation (SSF) of CCR, although the furans and organic acid inhibitors were very low. In this study, the high level of water-insoluble phenolic compounds such as 2-furoic acid, ferulic acid, p-coumaric acid, guaiacol, and p-hydroxybenzoic acid were detected in CCR and inhibited the growth and metabolism of Saccharomyces cerevisiae DQ1. An evolutionary adaptation strategy was developed by culturing the S. cerevisiae DQ1 strain in a series of media with the gradual increase of CCR hydrolysate. The high ethanol concentration (62.68g/L) and the yield (55.7%) were achieved in the SSF of CCR using the adapted S. cerevisiae DQ1. The results provided a practical method for improving performance of simultaneous saccharification and ethanol production from CCR.
工业废玉米芯残渣（CCR）富含纤维素，无需预处理即可直接水解。然而，在 CCR 的同步糖化和乙醇发酵（SSF）中，尽管呋喃和有机酸抑制剂含量很低，但经常观察到发酵性能不佳。在这项研究中，在 CCR 中检测到了高水平的水不溶性酚类化合物，如 2-糠酸、阿魏酸、对香豆酸、愈创木酚和对羟基苯甲酸，它们抑制了酿酒酵母 DQ1 的生长和代谢。通过在一系列含有逐渐增加的 CCR 水解物的培养基中培养酿酒酵母 DQ1 菌株，开发了一种进化适应策略。使用适应的酿酒酵母 DQ1 进行 CCR 的 SSF，可实现 62.68g/L 的高乙醇浓度（55.7%）和产率。结果为提高 CCR 的同步糖化和乙醇生产性能提供了一种实用方法。
