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通过两步酶解从脂质提取后的微藻生物质残渣中生产小球藻的脂质。

Lipid production of Chlorella vulgaris from lipid-extracted microalgal biomass residues through two-step enzymatic hydrolysis.

机构信息

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, PR China.

出版信息

Bioresour Technol. 2012 Aug;117:1-6. doi: 10.1016/j.biortech.2012.04.007. Epub 2012 Apr 10.

Abstract

Lipid-extracted microalgal biomass residues (LMBRs) were treated using cellulase, neutrase and alcalase in a two-step process and the resulting hydrolysates were used as a source of nutrients for the cultivation of Chlorella vulgaris under non-aerated and aerated conditions for lipid production. Aeration was favorable for cell growth and lipid accumulation and a biomass of approximately 3.28 g L(-1), lipid content of 35% and lipid productivity of 116 mg L(-1) d(-1) were obtained. Thus, the tested mode of LMBRs utilization was effective for nutrient recycling in microalgal biodiesel production.

摘要

采用两步法用纤维素酶、中性蛋白酶和碱性蛋白酶处理脂提取微藻生物质残渣(LMBRs),所得水解产物用作在非充气和充气条件下培养普通小球藻生产油脂的营养源。充气有利于细胞生长和脂质积累,获得了约 3.28 g/L 的生物量、35%的脂质含量和 116 mg/L/d 的脂质产率。因此,测试的 LMBRs 利用方式有效地实现了微藻生物柴油生产中的养分循环利用。

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