Khabour Omar F, Alzoubi Karem H, Mfady Doa'a S, Alasseiri Mohammed, Hasheesh Taghrid F
Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology Irbid, Jordan.
Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology Irbid, Jordan.
Int J Clin Exp Med. 2014 Apr 15;7(4):982-8. eCollection 2014.
The use of cisplatin in treatments of human malignancies is limited by its side effects that include DNA damage and the subsequent risk of developing secondary cancer. In this study, we examined the possible protective effect of Tempol against DNA damage induced by cisplatin in human lymphocytes using chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) assays. Cisplatin induced significant elevation in the frequencies of CAs and SCEs in cultured human lymphocytes (P < 0.01). Treatment of lymphocytes with Tempol significantly lowered CAs and SCEs induced by cisplatin. Tempol alone did not affect spontaneous levels of SCEs and CAs observed in the control group (P > 0.05). In conclusion, Tempol protects human lymphocytes against genotoxicity induced by the anticancer drug cisplatin.
顺铂在人类恶性肿瘤治疗中的应用受到其副作用的限制，这些副作用包括DNA损伤以及随后发生继发性癌症的风险。在本研究中，我们使用染色体畸变（CA）和姐妹染色单体交换（SCE）试验，检测了Tempol对顺铂诱导的人类淋巴细胞DNA损伤的可能保护作用。顺铂显著提高了培养的人类淋巴细胞中CA和SCE的频率（P < 0.01）。用Tempol处理淋巴细胞可显著降低顺铂诱导的CA和SCE。单独使用Tempol不影响对照组中观察到的SCE和CA的自发水平（P > 0.05）。总之，Tempol可保护人类淋巴细胞免受抗癌药物顺铂诱导的遗传毒性。
