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J Virol Methods. 2012 Sep;184(1-2):106-8. doi: 10.1016/j.jviromet.2012.05.007. Epub 2012 May 17.
Significant underquantitation of HIV RNA has been reported with the Roche Cobas AmpliPrep/Cobas TaqMan HIV Test (Version 1) compared to other assays. However, these studies have generally involved limited numbers of samples from select patient populations or analysis of samples that were undetectable in the TaqMan assay. Random plasma samples submitted from throughout the United States for HIV RNA quantitation (n=1263) were compared in the Roche TaqMan and Abbott RealTime assays. Twenty-four samples (1.9%) were discrepant, with a maximum difference between the two assays of 1.9logcopies/mL. These data indicate that both tests may be susceptible to underquantitation, but the incidence is low in this large cohort of samples from across the United States.
与其他检测方法相比，罗氏 Cobas AmpliPrep/Cobas TaqMan HIV 检测（第 1 版）报告的 HIV RNA 检测结果明显偏低。然而，这些研究通常涉及从特定患者人群中选择的有限数量的样本，或者对 TaqMan 检测不可检测的样本进行分析。对来自美国各地的用于 HIV RNA 定量的随机血浆样本（n=1263）进行了罗氏 TaqMan 和雅培实时检测的比较。24 个样本（1.9%）存在差异，两个检测方法之间的最大差异为 1.9logcopies/mL。这些数据表明，两种检测方法都可能存在检测结果偏低的情况，但在美国各地的大量样本中，这种情况的发生率较低。
