Laboratory of Botanic Mycology (UMR 5623 35), Virology and Industrial Microbiology, Faculty of Pharmaceutical Sciences, University Paul Sabatier Toulouse III, Toulouse, France.
J Med Food. 2012 Jul;15(7):671-6. doi: 10.1089/jmf.2011.0252. Epub 2012 May 21.
The volatile components from Croton campestris root bark were localized by an anatomical study and analyzed by gas chromatography-mass spectrometry for the first time. The roots of this plant showed secretory cells. These volatile constituents, isolated from the dichloromethane extract by hydrodistillation, were analyzed by gas chromatography-mass spectrometry. We found 69 components. They were characterized, and the major constituents of crude oil root barks were spathulenol (23.3%) and borneol (18.7%). Growth inhibitory activity of the active compounds in solution was evaluated by measuring minimal inhibitory concentrations using a broth micromethod. The minimal inhibitory concentration of root bark volatile constituents was 1.56 μg/mL for Staphylococcus aureus, 3.125 μg/mL for Candida albicans, and 6.25 μg/mL for Aspergillusniger.
首次通过解剖学研究定位了巴豆根皮中的挥发性成分,并通过气相色谱-质谱法对其进行了分析。该植物的根显示出分泌细胞。这些挥发性成分通过水蒸馏从二氯甲烷提取物中分离出来,并通过气相色谱-质谱法进行分析。我们发现了 69 种成分。对它们进行了表征,粗油根皮的主要成分是石竹烯(23.3%)和龙脑(18.7%)。通过使用肉汤微量法测量最小抑菌浓度来评估溶液中活性化合物的生长抑制活性。金黄色葡萄球菌的根皮挥发性成分的最小抑菌浓度为 1.56μg/mL,白色念珠菌为 3.125μg/mL,黑曲霉为 6.25μg/mL。