Departamento de Microbiología, Universidad de Córdoba, Córdoba, Spain.
FEMS Yeast Res. 2012 Sep;12(6):651-61. doi: 10.1111/j.1567-1364.2012.00815.x. Epub 2012 Jun 18.
In this work, we performed for the first time a proteomic approach to the processes induced by long-term potassium starvation in the halotolerant yeast Debaryomyces hansenii. The proteomic profile under this ionic stress conditions shows that important changes in gene expression take place as an adaptive response. We found a significant protein expression repression as well as metabolic changes such as the inhibition of the upper part of the glycolysis, the amino acid synthesis, and the Krebs cycle. On the other hand, genes related to stress responses, protein degradation, and sterols synthesis were upregulated in response to potassium deprivation. The findings in this study provide important information about how this particular yeast copes with ionic stress at molecular levels, which might further enrich the global understanding of salt tolerance processes in eukaryal systems and moreover highlighting the importance of the 'omics' approaches as a complement to the classical physiological studies.
在这项工作中，我们首次采用蛋白质组学方法研究了耐盐酵母德巴利氏酵母在长期低钾胁迫下诱导的过程。在这种离子胁迫条件下的蛋白质组图谱表明，重要的基因表达变化发生作为一种适应性反应。我们发现显著的蛋白质表达抑制以及代谢变化，如糖酵解的上半部分、氨基酸合成和克雷布斯循环的抑制。另一方面，与应激反应、蛋白质降解和固醇合成相关的基因被上调以响应钾的缺乏。这项研究的结果提供了关于特定酵母如何在分子水平上应对离子胁迫的重要信息，这可能进一步丰富真核系统耐盐过程的整体理解，并且突出了“组学”方法作为对经典生理研究的补充的重要性。
