Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Fungal Biol. 2013 Jun;117(6):399-410. doi: 10.1016/j.funbio.2013.04.004. Epub 2013 Apr 20.
Zinc plays a critical role in a diverse array of biochemical processes. However, an excess of zinc is deleterious to cells; therefore, cells require finely tuned homeostatic mechanisms to balance the uptake and the storage of zinc. There is also increasing evidence supporting the importance of zinc during infection. To understand better how Paracoccidioides adapts to zinc deprivation, we compared the two-dimensional (2D) gel protein profile of yeast cells during zinc starvation to yeast cells grown in a zinc rich condition. Protein spots were selected for comparative analysis based on the protein staining intensity, as determined by image analysis. In response to zinc deprivation, a total of 423 out of 845 protein spots showed a significant change in abundance. Quantitative RT-qPCR analysis of RNA from Paracoccidioides grown under zinc restricted conditions validated the correlation between the differentially regulated proteins and transcripts. According to the proteomic data, zinc deficiency may be a stressor to Paracoccidioides, as suggested by the upregulation of a number of proteins related to stress response, cell rescue, and virulence. Other process induced by zinc deprivation included gluconeogenesis. Conversely, the methylcitrate cycle was downregulated. Overall, the results indicate a remodelling of the Paracoccidioides response to the probable oxidative stress induced during zinc deprivation.
锌在多种生化过程中起着至关重要的作用。然而，过量的锌对细胞是有害的；因此，细胞需要精细的动态平衡机制来平衡锌的摄取和储存。越来越多的证据也支持锌在感染过程中的重要性。为了更好地理解球孢子菌如何适应锌缺乏，我们比较了在缺锌条件下酵母细胞和在富含锌条件下生长的酵母细胞的二维（2D）凝胶蛋白图谱。根据图像分析确定的蛋白染色强度，选择蛋白斑点进行比较分析。在对锌的剥夺的响应中，845 个蛋白斑点中共有 423 个蛋白斑点的丰度发生了显著变化。在限制锌条件下生长的球孢子菌的 RNA 的定量 RT-qPCR 分析验证了差异调节蛋白和转录物之间的相关性。根据蛋白质组学数据，锌缺乏可能是球孢子菌的一种应激源，因为许多与应激反应、细胞挽救和毒力相关的蛋白上调。锌剥夺诱导的其他过程包括糖异生。相反，甲基柠檬酸循环被下调。总的来说，这些结果表明球孢子菌对可能在锌缺乏诱导的氧化应激的反应发生了重塑。
