Bonnafous J C, Dornand J, Mani J C
Biochim Biophys Acta. 1979 Oct 4;587(2):180-91. doi: 10.1016/0304-4165(79)90352-0.
Adenosine-cyclic AMP relationships have been studied in pig mesenteric lymph node lymphocytes. The early 2--3-fold increase in cyclic AMP accumulation elicited by adenosine and 2-chloroadenosine, an adenosine deaminase-resistant analogue, could not be correlated to similar effects on the adenylate cyclase activity of disrupted cell preparations, but rather to the competitive inhibition of the low Km (0.17 muM) cyclic AMP phosphodiesterase. The existence of adenosine receptors coupled to lymphocyte adenylate cyclase, which had been proposed by several authors, could not be confirmed by this study Adenosine-cyclic AMP relationships do not appear to be involved in concanavalin A stimulation of pig lymphocytes.
已对猪肠系膜淋巴结淋巴细胞中的腺苷 - 环磷酸腺苷关系进行了研究。腺苷和2 - 氯腺苷（一种腺苷脱氨酶抗性类似物）引发的环磷酸腺苷积累早期2至3倍的增加，与对破碎细胞制剂的腺苷酸环化酶活性的类似影响无关，而是与低Km（0.17μM）环磷酸腺苷磷酸二酯酶的竞争性抑制有关。几位作者提出的与淋巴细胞腺苷酸环化酶偶联的腺苷受体的存在，本研究无法证实。腺苷 - 环磷酸腺苷关系似乎不参与伴刀豆球蛋白A对猪淋巴细胞的刺激。
