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利用电子显微镜和酶联免疫吸附测定法检测新生犊牛腹泻中的轮状病毒。

Use of electron microscopy and an enzyme-linked immunosorbent assay for the detection of rotaviruses in neonatal calf diarrhea.

作者信息

Payment P, Marsolais G, Trudel M, Fauvel M, Lamontagne L, Assaf R, Marois P

出版信息

Can J Comp Med. 1979 Jul;43(3):328-9.

PMID:226249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1319897/
Abstract

Electron microscopy and an enzyme-linked immunosorbent assay were compared for the diagnosis of rotaviruses associated with neonatal calf diarrhea. One hundred percent correlation was observed when 125 samples were tested by both techniques. Both techniques were equally efficient in detecting rotaviruses. The enzyme-linked immunosorbent assay was more sensitive but being specific could not detect other viruses.

摘要

对电子显微镜检查和酶联免疫吸附测定法进行了比较,以诊断与新生小牛腹泻相关的轮状病毒。当用这两种技术对125份样本进行检测时,观察到两者的相关性为100%。两种技术在检测轮状病毒方面同样有效。酶联免疫吸附测定法更敏感,但因其具有特异性,无法检测出其他病毒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2790/1319897/b04d15ddc2ef/compmed00027-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2790/1319897/b04d15ddc2ef/compmed00027-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2790/1319897/b04d15ddc2ef/compmed00027-0097-a.jpg

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Use of electron microscopy and an enzyme-linked immunosorbent assay for the detection of rotaviruses in neonatal calf diarrhea.利用电子显微镜和酶联免疫吸附测定法检测新生犊牛腹泻中的轮状病毒。
Can J Comp Med. 1979 Jul;43(3):328-9.
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本文引用的文献

1
Neonatal calf diarrhea: purification and electron microscopy of a coronavirus-like agent.新生小牛腹泻:一种类冠状病毒病原体的纯化及电子显微镜观察
Am J Vet Res. 1972 Jun;33(6):1147-56.
2
Purification, morphology and partial characterization of a reovirus-like agent associated with neonatal calf diarrhea.与新生犊牛腹泻相关的呼肠孤病毒样病原体的纯化、形态学及部分特性分析
Can J Comp Med. 1971 Jul;35(3):195-202.
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Specific identification of enteroviruses by immuno-electron microscopy using a serum-in-agar diffusion method.
Can J Microbiol. 1973 May;19(5):585-9. doi: 10.1139/m73-096.
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Quantitative enzyme immunoassay (ELISA) in microbiology.微生物学中的定量酶免疫测定(ELISA)。
Med Biol. 1977 Aug;55(4):193-200.
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Diagnosis of viral agents associated with neonatal calf diarrhea.与新生犊牛腹泻相关病毒病原体的诊断
Can J Comp Med. 1978 Apr;42(2):168-71.
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Hemagglutination and hemagglutination-inhibition studies with a strain of Nebraska calf diarrhea virus (bovine rotavirus).
Intervirology. 1978;9(2):95-105. doi: 10.1159/000148927.
7
Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves.用于诊断犊牛轮状病毒感染的酶联免疫吸附测定法。
J Clin Microbiol. 1977 Nov;6(5):530-2. doi: 10.1128/jcm.6.5.530-532.1977.
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Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis.用于检测婴幼儿肠胃炎人呼肠孤病毒样病原体的酶联免疫吸附测定(ELISA)
Lancet. 1977 Aug 6;2(8032):263-7. doi: 10.1016/s0140-6736(77)90951-5.