用于检测婴幼儿肠胃炎人呼肠孤病毒样病原体的酶联免疫吸附测定（ELISA）

Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis.

作者信息

Yolken R H, Kim H W, Clem T, Wyatt R G, Kalica A R, Chanock R M, Kapikian A Z

出版信息

Lancet. 1977 Aug 6;2(8032):263-7. doi: 10.1016/s0140-6736(77)90951-5.

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the human reovirus-like agent of infantile gastroenteritis in human stools. The results of the assay can be read either with a simple colorimeter or the naked eye. Investigations with 143 samples from children with gastroenteritis and 75 samples from children with other illnesses showed that the ELISA was as sensitive as electron microscopy or radioimmunoassay for detection of this agent. In addition, the ELISA was simple to perform and, when read visually, did not require sophisticated technical equipment. These advantages make it suitable for field work.

摘要

已开发出一种酶联免疫吸附测定法（ELISA），用于检测人类粪便中引起婴儿肠胃炎的呼肠孤病毒样病原体。该测定结果既可用简单的比色计读取，也可用肉眼读取。对143份肠胃炎患儿样本和75份其他疾病患儿样本的研究表明，ELISA在检测该病原体方面与电子显微镜检查或放射免疫测定法一样灵敏。此外，ELISA操作简便，肉眼读取结果时无需复杂的技术设备。这些优点使其适用于现场工作。