Department of Biochemistry, Oxford Centre for Integrative Systems Biology, University of Oxford, Oxford, United Kingdom.
PLoS One. 2012;7(5):e37737. doi: 10.1371/journal.pone.0037737. Epub 2012 May 22.
Phosphosignalling pathways are an attractive option for the synthetic biologist looking for a wide repertoire of modular components from which to build. We demonstrate that two-component systems can be used in synthetic biology. However, their potential is limited by the fact that host cells contain many of their own phosphosignalling pathways and these may interact with, and cross-talk to, the introduced synthetic components. In this paper we also demonstrate a simple bioinformatic tool that can help predict whether interspecies cross-talk between introduced and native two-component signalling pathways will occur and show both in vitro and in vivo that the predicted interactions do take place. The ability to predict potential cross-talk prior to designing and constructing novel pathways or choosing a host organism is essential for the promise that phosphosignalling components hold for synthetic biology to be realised.
磷酸信号通路是合成生物学家寻找广泛的模块化组件的理想选择，这些组件可以用来构建。我们证明了可以在合成生物学中使用双组分系统。然而，它们的潜力受到宿主细胞中存在许多自身磷酸信号通路的限制，这些通路可能与引入的合成组件相互作用和串扰。在本文中，我们还展示了一种简单的生物信息学工具，可以帮助预测引入的和本地的双组分信号通路之间是否会发生种间串扰，并在体外和体内显示预测的相互作用确实会发生。在设计和构建新的通路或选择宿主生物之前，预测潜在的串扰的能力对于实现磷酸信号组件在合成生物学中的应用至关重要。
