Ondokuz Mayıs University, Faculty of Agriculture, Department of Agricultural Biotechnology, Samsun, Turkey.
J Enzyme Inhib Med Chem. 2013 Aug;28(4):824-9. doi: 10.3109/14756366.2012.688042. Epub 2012 May 28.
Cord blood has numerous facilities for life and used in many different areas. Cord blood contains many different catalytic proteins including antioxidant enzymes. Here we purified human cord blood glutathione reductase (hcbGR), glutathione S-transferase (hcbGST) and human cord blood glutathione peroxidase (hcbGPx) from human cord blood erythrocytes and analyzed the inhibition effects of the antibiotics incorporating cefuroxime, ceftriaxone, ceftizoxime and cefoperazone, on these enzymes. K(I) values for the drugs ranged from 10.42 to 28.72 µM for hcbGR, 32.7 to 244.8 µM for hcbGPx, and 32.39 to 267.3 µM for hcbGST. Cefuroxime caused the highest inhibition on all enzymes with KI values of 10.42, 32.39, 32.7 µM for hcbGR, hcbGST, and hcbGPx, respectively. All drugs displayed non-competitive inhibition regardless of their structures. Since these drugs are often used during pregnancy, identification of possible undesired impacts on various parameters has a great importance for pharmacological and medical applications.
脐带血具有许多生命支持功能，可应用于许多不同领域。脐带血中含有许多不同的催化蛋白，包括抗氧化酶。在这里，我们从人脐带血红细胞中纯化了人脐带血谷胱甘肽还原酶（hcbGR）、谷胱甘肽 S-转移酶（hcbGST）和人脐带血谷胱甘肽过氧化物酶（hcbGPx），并分析了包含头孢呋辛、头孢曲松、头孢唑肟和头孢哌酮的抗生素对这些酶的抑制作用。这些药物对 hcbGR 的 K(I) 值范围为 10.42 至 28.72 μM，对 hcbGPx 的 K(I) 值范围为 32.7 至 244.8 μM，对 hcbGST 的 K(I) 值范围为 32.39 至 267.3 μM。头孢呋辛对所有酶的抑制作用最强，其对 hcbGR、hcbGST 和 hcbGPx 的 KI 值分别为 10.42、32.39 和 32.7 μM。所有药物均显示非竞争性抑制，无论其结构如何。由于这些药物在怀孕期间经常使用，因此确定它们对各种参数可能产生的不良影响对于药理学和医学应用具有重要意义。
