Faculty of Agriculture, Department of Agricultural Biotechnology, Ondokuz Mayıs University, Samsun, 55139, Turkey.
Protein J. 2023 Aug;42(4):421-426. doi: 10.1007/s10930-023-10104-w. Epub 2023 Mar 15.
Glutathione S-Transferase (GST) enzyme is abundant in mammals, insects, fish and microorganisms, as well as in various tissues of these species, particularly in tissues exposed to xenobiotics from the environment. As a result, the enzyme execute detoxifying function by scavenging a diverse range of xenobiotics, such as chemotherapeutic medicines, environmental carcinogens and endogenous compounds. In this study, GST enzyme was partially purified from mallow (Malva slyvestris L.) seed for the first time and the kinetic parameters were determined. The optimum ionic intensity was found in 400 mM Tris-Buffer, optimum pH: 7.0, and optimum substrate concentration was determined as 0.2 mM. One of the biggest reasons for deterioration of ecological balance in nature is heavy metal accumulation in soil, air and water which becomes a major threat to the vital activities of living things. In this study, inhibitory effects of Cd, Ag, Zn and Fe heavy metals, which are common in nature, on mallow seed glutathione S-transferase enzyme were investigated. Each heavy metal showed micromolar inhibitory effects on enzyme activity. IC values of the metals were calculated as 60.93, 74.602, 178.22 and 369 µM, respectively.
谷胱甘肽 S-转移酶（GST）酶在哺乳动物、昆虫、鱼类和微生物中以及这些物种的各种组织中都很丰富，特别是在暴露于环境中异源生物的组织中。因此，该酶通过清除各种异源生物（如化疗药物、环境致癌物和内源性化合物）来执行解毒功能。在这项研究中，首次从锦葵（Malva sylvestris L.）种子中部分纯化 GST 酶，并确定了其动力学参数。在 400mM Tris 缓冲液中发现最佳离子强度，最佳 pH 值为 7.0，最佳底物浓度确定为 0.2mM。造成自然界生态平衡恶化的最大原因之一是土壤、空气和水中重金属的积累，这对生物的生命活动构成了重大威胁。在这项研究中，研究了在自然界中常见的 Cd、Ag、Zn 和 Fe 重金属对锦葵种子谷胱甘肽 S-转移酶的抑制作用。每种重金属对酶活性都表现出微摩尔抑制作用。这些金属的 IC 值分别计算为 60.93、74.602、178.22 和 369µM。
