Udan Ryan S, Dickinson Mary E
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas, USA.
Methods Enzymol. 2010;476:329-49. doi: 10.1016/S0076-6879(10)76019-5.
For the past three decades, methods for culturing mouse embryos ex vivo have been optimized in order to improve embryo viability and physiology throughout critical stages of embryogenesis. Combining advances made in the production of transgenic animals and in the development of different varieties of fluorescent proteins (FPs), time-lapse imaging is becoming more and more popular in the analysis of dynamic events during mouse development. Targeting FPs to specific cell types or subcellular compartments has enabled researchers to study cell proliferation, apoptosis, migration, and changes in cell morphology in living mouse embryos in real time. Here we provide a guide for time-lapse imaging of early stages of mouse embryo development.
在过去三十年里,为提高胚胎在胚胎发育关键阶段的活力和生理机能,小鼠胚胎体外培养方法不断优化。结合转基因动物生产及不同种类荧光蛋白(FPs)开发方面取得的进展,延时成像在分析小鼠发育过程中的动态事件时越来越受欢迎。将荧光蛋白靶向特定细胞类型或亚细胞区室,使研究人员能够实时研究活体小鼠胚胎中的细胞增殖、凋亡、迁移以及细胞形态变化。在此,我们提供一份小鼠胚胎发育早期延时成像指南。