Hyink Deborah
Department of Medicine, Baylor College of Medicine, Houston, TX, USA.
Methods Mol Biol. 2012;886:87-93. doi: 10.1007/978-1-61779-851-1_8.
Three-dimensional imaging is a valuable tool for analyzing kidney growth and development. This technique provides information about spatial relationships between the branching ureteric bud, nephrons, and other structures within the kidney. Availability of user-friendly volume-rendering software now puts this technique within the capability of most laboratories with access to a confocal microscope. This paper describes how to prepare samples and acquire images and three-dimensional volume-rendered images.
三维成像技术是分析肾脏生长发育的一项重要工具。该技术可提供有关分支输尿管芽、肾单位及肾脏内其他结构之间空间关系的信息。如今，用户友好型容积再现软件的出现使大多数配备共聚焦显微镜的实验室都能够使用这项技术。本文介绍了如何制备样本、采集图像以及生成三维容积再现图像。
