Department of Mechanical Engineering, Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, MI, USA.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2012 Jul-Aug;4(4):414-27. doi: 10.1002/wnan.1175. Epub 2012 May 25.
Stem cells possess the ability of self-renewal and differentiation into specific cell types. Therefore, stem cells have great potentials in fundamental biology studies and clinical applications. The most urgent desire for stem cell research is to generate appropriate artificial stem cell culture system, which can mimic the dynamic complexity and precise regulation of the in vivo biochemical and biomechanical signals, to regulate and direct stem cell behaviors. Precise control and regulation of the biochemical and biomechanical stimuli to stem cells have been successfully achieved using emerging micro/nanoengineering techniques. This review provides insights into how these micro/nanoengineering approaches, particularly microcontact printing and elastomeric micropost array, are applied to create dynamic and complex environment for stem cells culture.
干细胞具有自我更新和分化为特定细胞类型的能力。因此，干细胞在基础生物学研究和临床应用中有很大的潜力。干细胞研究最迫切的愿望是产生合适的人工干细胞培养系统，该系统可以模拟体内生化和生物力学信号的动态复杂性和精确调节，从而调节和指导干细胞行为。利用新兴的微/纳工程技术，可以成功地对干细胞的生化和生物力学刺激进行精确控制和调节。本综述深入探讨了这些微/纳工程方法，特别是微接触印刷和弹性微柱阵列，如何应用于创造用于干细胞培养的动态复杂环境。
