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《水稻蛋白基因组学数据库 OryzaPG-DB:发展、扩展及新特性》

The Rice Proteogenomics Database OryzaPG-DB: Development, Expansion, and New Features.

机构信息

Institute for Advanced Biosciences, Keio University Tokyo, Japan.

出版信息

Front Plant Sci. 2012 Apr 2;3:65. doi: 10.3389/fpls.2012.00065. eCollection 2012.

Abstract

Our recently developed rice proteogenomics database (OryzaPG-DB) is the first sustainable resource for rice shotgun-based proteogenomics, providing information on peptides identified in rice protein digested peptides measured by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS), and mapping of the peptides to their genomic origins and the genomic novelty of each peptide. The sequences of the peptides, proteins, cDNAs and genes, and the gene annotations are available for download in FASTA and GFF3 formats, respectively. Further, an annotated visualization of the gene models, corresponding peptides, and genomic novelty is available for each gene, and MS/MS spectra are available for each peptide. In this article, we discuss the utilization of OryzaPG-DB and report on its development, recent content expansions, and newly added features in the current version (OryzaPG-DB v1.1).

摘要

我们最近开发的水稻蛋白基因组学数据库(OryzaPG-DB)是第一个基于水稻鸟枪法的蛋白基因组学可持续资源,提供了通过液相色谱-串联质谱(LC-MS/MS)测量的水稻蛋白消化肽中鉴定的肽的信息,并将肽映射到其基因组起源和每个肽的基因组新颖性。肽、蛋白质、cDNA 和基因的序列以及基因注释分别以 FASTA 和 GFF3 格式提供下载。此外,每个基因都提供了基因模型、相应肽和基因组新颖性的注释可视化,每个肽都提供了 MS/MS 谱。在本文中,我们讨论了 OryzaPG-DB 的利用,并报告了它的开发、最近的内容扩展以及当前版本(OryzaPG-DB v1.1)中新增的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0256/3355581/71b819b09446/fpls-03-00065-g001.jpg

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