大鼠基因组中具有限制性内切酶Taq I切割位点的重复序列。

Repetitive sequences in the rat genome that have cleavage sites for the restriction endonuclease Taq I.

作者信息

Kikuchi H, Sekiya T, Nishimura S, Watanabe M

机构信息

Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan.

出版信息

Tohoku J Exp Med. 1990 Jun;161(2):91-9. doi: 10.1620/tjem.161.91.

DOI:10.1620/tjem.161.91

PMID:2264064

Abstract

A cleavage of rat DNA with restriction endonuclease Taq I produced at least 8 discrete bands. One of the bands, 0.29 kilobase pairs (kb) long, was isolated and cloned in plasmid. The hybridization of this fragment to genomic DNA digested with Taq I showed 1.6 kb, 1.2 kb and 0.29 kb band in a smear hybridization signal. A repetition of the DNA sequence of this fragment was about 12,500 copies/haploid genome of both Buffalo and Sprague-Dawley rats by the Cot-hybridization analysis. Four independent clones of 0.29 kb Taq I fragment share high homology (97%).

摘要

用限制性内切酶Taq I切割大鼠DNA产生了至少8条离散条带。其中一条长度为0.29千碱基对（kb）的条带被分离出来并克隆到质粒中。该片段与用Taq I消化的基因组DNA杂交，在涂片杂交信号中显示出1.6 kb、1.2 kb和0.29 kb的条带。通过Cot杂交分析，该片段的DNA序列在布法罗大鼠和斯普拉格-道利大鼠的单倍体基因组中重复约12,500次。0.29 kb Taq I片段的四个独立克隆具有高度同源性（97%）。