Manuelidis L, Biro P A
Nucleic Acids Res. 1982 May 25;10(10):3221-39. doi: 10.1093/nar/10.10.3221.
The genomic representation and organization of sequences homologous to a cloned Hind III 1.9 kb repeated DNA fragment were studied. Approximately 80% of homologous repeated DNA was contained in a genomic Hind III cleavage band of 1.9 kb. Double digestion studies indicated that the genomic family, in the majority, followed the arrangement of the sequenced clone, with minor restriction cleavage variations compatible with a few base changes. Common restriction sites external to the 1.9 kb sequence were mapped, and hybridization of segments of the cloned sequence indicated the 1.9 kb DNA was itself not tandemly repeated. Kpn I bands which were homologous to the sequence contained specific regions of the repeat, and the molecular weight of these larger fragments could be simply explained. Mapping of common external restriction sites indicated that in some but not all cases the repeat could be organized in larger defined blocks of greater than or equal to 5.5 kb. In some instances, flanking regions adjacent to the repeat may contain common DNA elements such as other repeated DNA sequences, or possibly rearranged segments of the 1.9 kb sequence. It is suggested that although the 1.9 kb sequence is not strictly contiguous, at least some of these repeated sequences in the human genome are arranged in clustered or intercalary arrays. A region of the 1.9 kb sequence hybridized to a mouse repeated DNA, indicating homology beyond the primates.
对与克隆的Hind III 1.9 kb重复DNA片段同源的序列的基因组表示和组织进行了研究。大约80%的同源重复DNA包含在一个1.9 kb的基因组Hind III切割带中。双酶切研究表明，基因组家族在大多数情况下遵循测序克隆的排列方式，只有少量限制性切割变异与少数碱基变化相符。绘制了1.9 kb序列外部的常见限制性位点图谱，克隆序列片段的杂交表明1.9 kb DNA本身不是串联重复的。与该序列同源的Kpn I条带包含重复序列的特定区域，这些较大片段的分子量可以得到简单解释。常见外部限制性位点的图谱表明，在某些但不是所有情况下，重复序列可以组织成大于或等于5.5 kb的更大确定片段。在某些情况下，与重复序列相邻的侧翼区域可能包含常见的DNA元件，如其他重复DNA序列，或可能是1.9 kb序列的重排片段。有人提出，虽然1.9 kb序列并非严格连续，但人类基因组中的至少一些这些重复序列是以簇状或间插阵列的形式排列的。1.9 kb序列的一个区域与小鼠重复DNA杂交，表明其同源性超出了灵长类动物。
