Decoville M, Malfoy B, Locker D
Centre de Sélection et d'Elevage des Animaux, Université d'Orléans, France.
Eur J Biochem. 1988 Jul 1;174(4):679-84. doi: 10.1111/j.1432-1033.1988.tb14151.x.
A new middle repetitive sequence is described in the mouse genome. It has been revealed with a recombinant clone isolated from a Mus musculus BamHI gene library constructed in pBR322 and containing an insertion of 1.73 kb. When digests of genomic DNA were subjected to Southern blot hybridization, using the 1.73-kb insert as probe, we obtained a light smear and discrete bands, indicating a dispersion in the mouse genome of this sequence. This 1.73-kb sequence seems to be a part of a greater repetitive sequence at least 6 kb in length. The sizes of the bands hybridizing with the 1.73-kb insert are similar when compared between different laboratory strains but differ remarkably between the two species M. musculus and Mus caroli. We have shown also a great variation in the copy number of the sequence studied between these two species. When rat DNA is probed with the 1.73-kb insert, no hybridization is observed. Subcloning of the 1.73-kb sequence in three fragments has pointed out that the reiteration was not homogeneous along the 1.73-kb sequence. The 1.73-kb clone was sequenced and compared with other interspersed repetitive sequences, previously described in the rodent genome, and no homology was found.
在小鼠基因组中发现了一种新的中度重复序列。它是通过从构建于pBR322的小家鼠BamHI基因文库中分离出的一个重组克隆揭示的，该克隆含有一个1.73 kb的插入片段。当用基因组DNA酶切产物进行Southern印迹杂交，以1.73 kb的插入片段为探针时，我们得到了一条淡淡的拖尾和离散条带，表明该序列在小鼠基因组中呈分散分布。这个1.73 kb的序列似乎是一个更长的至少6 kb的重复序列的一部分。与1.73 kb插入片段杂交的条带大小在不同实验室品系之间比较时相似，但在小家鼠和卡罗来鼠这两个物种之间差异显著。我们还表明，这两个物种之间所研究序列的拷贝数有很大差异。用1.73 kb插入片段探测大鼠DNA时，未观察到杂交现象。将1.73 kb序列亚克隆为三个片段表明，重复序列在1.73 kb序列上并非均匀分布。对1.73 kb克隆进行了测序，并与先前在啮齿动物基因组中描述的其他散布重复序列进行了比较，未发现同源性。
