从牛子宫内膜分离的单核细胞的增殖反应及TCGF的产生。

Blastogenic responses and production of TCGF by mononuclear cells isolated from the bovine endometrium.

作者信息

Watson E D, Zanecosky H G

机构信息

Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348.

出版信息

Vet Immunol Immunopathol. 1990 Nov;26(3):227-36. doi: 10.1016/0165-2427(90)90093-8.

DOI:10.1016/0165-2427(90)90093-8

PMID:2264286

Abstract

Mononuclear cells (MNCs) were isolated from the endometrium of cattle by enzymatic digestion followed by centrifugation on a density gradient. Of the cells isolated, 32 +/- 3.2% rosetted AET-treated sheep red blood cells, 9 +/- 0.4% possessed surface membrane immunoglobulin, and 15 +/- 6.1% were esterase positive. The cells possessed the ability to proliferate in the presence of T cell mitogens and produced T cell growth factor (TCGF) after in vitro stimulation with Concanavalin A. Endometrial MNCs did not appear to suppress the blastogenesis of peripheral blood MNCs.

摘要

通过酶消化，然后在密度梯度上离心，从牛的子宫内膜中分离出单核细胞（MNCs）。在分离出的细胞中，32±3.2%的细胞与经AET处理的绵羊红细胞形成玫瑰花结，9±0.4%的细胞具有表面膜免疫球蛋白，15±6.1%的细胞酯酶呈阳性。这些细胞在T细胞有丝分裂原存在的情况下具有增殖能力，在用刀豆球蛋白A进行体外刺激后产生T细胞生长因子（TCGF）。子宫内膜MNCs似乎不会抑制外周血MNCs的增殖。