Quesada J R, Murphy S G
Int Arch Allergy Appl Immunol. 1982;68(2):138-43. doi: 10.1159/000233083.
Alpha-naphthyl-acetate-esterase (ANAE) activity was histochemically demonstrated in human T lymphocytes and their subpopulations. Examination of T lymphocytes bearing Fc receptors for IgM showed the presence of circumscribed deposits of esterase activity, while T cells with FC receptors for IgG showed of predominantly diffuse granular pattern. The difference in the topography of the enzymatic activity was shown to be related to cellular activation resulting from exposure to IgG-sensitized bovine erythrocytes or to strong polyclonal activation by lectin mitogens. The ANAE stain also characterized monocytes, which exhibited an intense nongranular cytoplasmic stain. Non-T cells showed no detectable ANAE activity when tested by this method.
采用组织化学方法证实了α-萘乙酸酯酶（ANAE）活性在人T淋巴细胞及其亚群中的存在。对带有IgM Fc受体的T淋巴细胞进行检测发现，有局灶性酯酶活性沉积物，而带有IgG Fc受体的T细胞则主要呈现弥漫性颗粒状模式。酶活性的拓扑结构差异与暴露于IgG致敏的牛红细胞后引起的细胞活化或凝集素丝裂原引起的强烈多克隆活化有关。ANAE染色还可对单核细胞进行表征，单核细胞呈现强烈的非颗粒状胞质染色。用此方法检测时，非T细胞未显示出可检测到的ANAE活性。
