Department of Pathology and Pathological Physiology, Medical College, Jinan University, Guangzhou 510632, People's Republic of China.
J Physiol Biochem. 2012 Dec;68(4):603-10. doi: 10.1007/s13105-012-0180-0. Epub 2012 May 29.
To maintain islets survival and function is critical in successful pancreatic transplantation. Pancreatic progenitors cells (PPCs) with lineage potentials, giving rise to exocrine, endocrine, and duct cells, reside in developing and adult pancreas. As tissue-specific stem cells, they can produce pancreatic tissue-specific matrix factors to promote islets survival and function. The aim of our research was to investigate the protective effect of rat pancreatic-duodenal homeobox 1 (Pdx1)(+)/nestin(+) PPCs on islets. In vitro, co-culturing islets with Pdx1(+)/nestin(+) PPCs prolonged the former survival from 7 to 14 days. Furthermore, with high glucose (300.8 mg/dl) stimuli, the yield of insulin in co-cultures was significantly higher than that in control group (single islets group). In vivo, co-transplanting islets and Pdx1(+)/nestin(+) PPCs for 3 days, the blood glucose of diabetic rat was significantly decreased to normal level and sustained for 2 weeks. Without Pdx1(+)/nestin(+) PPCs in islets transplantation, hyperglycemia was reversed at day 7 and recovered at day 15. Pathology analysis showed that islets had remnants in co-transplantation at day 21, as complete graft rejection in alone islets transplantation. Our study showed that Pdx1(+)/nestin(+) PPCs displayed the ability of preserving islets viability and function in vitro and prolonging their survival in vivo.
维持胰岛的存活和功能对于成功的胰腺移植至关重要。具有谱系潜力的胰腺祖细胞 (PPCs) 可产生外分泌、内分泌和导管细胞，存在于发育中和成年的胰腺中。作为组织特异性干细胞，它们可以产生胰腺组织特异性基质因子，以促进胰岛的存活和功能。我们的研究旨在探讨大鼠胰腺十二指肠同源盒 1 (Pdx1)(+) /巢蛋白(+) PPCs 对胰岛的保护作用。在体外，与 Pdx1(+) /巢蛋白(+) PPCs 共培养可将胰岛的存活时间从 7 天延长至 14 天。此外，在高葡萄糖（300.8mg/dl）刺激下，共培养物中胰岛素的产量明显高于对照组（单个胰岛组）。在体内，共移植胰岛和 Pdx1(+) /巢蛋白(+) PPCs 3 天，糖尿病大鼠的血糖明显降至正常水平并持续 2 周。在没有 Pdx1(+) /巢蛋白(+) PPCs 的胰岛移植中，高血糖在第 7 天逆转，并在第 15 天恢复。病理分析显示，在共移植中，胰岛在第 21 天仍有残留，而在单独胰岛移植中则完全排斥。我们的研究表明，Pdx1(+) /巢蛋白(+) PPCs 在体外具有维持胰岛活力和功能的能力，并在体内延长其存活时间。
