Naju Foundation of Natural Dyeing Culture, Naju 520-931, South Korea.
Appl Biochem Biotechnol. 2012 Aug;167(7):1986-2004. doi: 10.1007/s12010-012-9723-7. Epub 2012 May 29.
Seeds and leaves of indigo (Polygonum tinctorium Ait.) plant were investigated and compared with another medicinal plant named prolipid for their properties such as chemical composition, antioxidant, and anticancer effects by Fourier transform infrared, three-dimensional fluorescence spectroscopy, and electrospray ionization-MS in negative mode. It was found that polyphenols, flavonoids, and flavanols were significantly higher in prolipid (P<0.05), following by indigo mature leaves, immature leaves, and seeds. Methanol extract of mature indigo leaves in comparison with the ethyl acetate extract showed higher inhibition of proliferation. The interaction between polyphenol extracts of indigo mature leaves and BSA showed that indigo has a strong ability, as other widely used medicinal plants, to quench the intrinsic fluorescence of BSA by forming complexes. In conclusion, indigo mature leaves were compared with prolipid. High content of bioactive compounds, antioxidant, fluorescence, and antiproliferative properties of indigo justifies the use of this plant as a medicinal plant and a new source of antioxidants.
对菘蓝（Polygonum tinctorium Ait.）的种子和叶子进行了研究，并将其与另一种名为脂溶性的药用植物进行了比较，比较其化学组成、抗氧化和抗癌作用，采用傅里叶变换红外光谱、三维荧光光谱和电喷雾电离-MS 在负离子模式下。结果表明，脂溶性中多酚、类黄酮和黄烷醇的含量显著高于靛蓝（P<0.05），其次是成熟叶、未成熟叶和种子。与乙酸乙酯提取物相比，成熟靛蓝叶的甲醇提取物表现出更高的增殖抑制作用。靛蓝成熟叶多酚提取物与 BSA 的相互作用表明，靛蓝具有很强的能力，就像其他广泛使用的药用植物一样，通过形成复合物来猝灭 BSA 的固有荧光。总之，将成熟的靛蓝叶与脂溶性进行了比较。高含量的生物活性化合物、抗氧化、荧光和抗增殖特性证明了该植物作为药用植物和抗氧化剂新来源的合理性。
