Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Curr Microbiol. 2012 Sep;65(3):231-6. doi: 10.1007/s00284-012-0150-3. Epub 2012 May 29.
A bacterial strain P2 capable of degrading 3,5,6-trichloro-2-pyridinol (TCP) was isolated and characterized. Phylogenetic analysis based on 16S rRNA gene sequence indicated that it belonged to the genus of Cupriavidus, because it showed the highest sequence similarity to Cupriavidus pauculus LMG 3413(T) (99.7 %) and DNA-DNA relatedness value between strain P2 and C. pauculus LMG 3413(T) was 76.8 %. In combination with morphological, physiological and biochemical characters, strain P2 was identified as C. pauculus. It could use TCP as the sole carbon source and energy source for its growth. It showed a high average degradation rate of 10 mg/L h in mineral salt medium amended with TCP (50-800 mg/L). During TCP degradation, chloridion was released into the medium in two obvious discontinuous stages. Along with this, two colorful metabolites were produced. Finally, the molarity of the total released chloridion was three times that of the initial TCP in the medium. This is the first report of TCP-degrading strain from the genus of Cupriavidus and the detection of two colorful metabolites during TCP degradation. Strain P2 might be a promising candidate for its application in the bioremediation of TCP-polluted environments.
一株能够降解 3,5,6-三氯-2-吡啶醇（TCP）的细菌菌株 P2 被分离并鉴定。基于 16S rRNA 基因序列的系统发育分析表明，它属于 Cupriavidus 属，因为它与 Cupriavidus pauculus LMG 3413(T) 的序列相似性最高（99.7%），且菌株 P2 与 C. pauculus LMG 3413(T) 的 DNA-DNA 同源性值为 76.8%。结合形态学、生理学和生物化学特征，菌株 P2 被鉴定为 C. pauculus。它可以将 TCP 作为唯一的碳源和能源用于生长。在添加 TCP（50-800 mg/L）的无机盐培养基中，它表现出 10 mg/L h 的高平均降解率。在 TCP 降解过程中，氯离子分两个明显的不连续阶段释放到培养基中。同时，生成了两种有颜色的代谢物。最后，总释放的氯离子摩尔浓度是培养基中初始 TCP 的三倍。这是首次从 Cupriavidus 属中报道 TCP 降解菌株，并在 TCP 降解过程中检测到两种有颜色的代谢物。菌株 P2 可能是在 TCP 污染环境的生物修复中应用的有前途的候选菌株。
