Qu Yuanyuan, Shen E, Ma Qiao, Zhang Zhaojing, Liu Ziyan, Shen Wenli, Wang Jingwei, Li Duanxing, Li Huijie, Zhou Jiti
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
J Environ Sci (China). 2015 Aug 1;34:126-32. doi: 10.1016/j.jes.2015.01.023. Epub 2015 May 26.
Indole, a typical nitrogen heterocyclic aromatic pollutant, is extensively spread in industrial wastewater. Microbial degradation has been proven to be a feasible approach to remove indole, whereas the microbial resources are fairly limited. A bacterial strain designated as SHE was isolated and found to be an efficient indole degrader. It was identified as Cupriavidus sp. according to 16S rRNA gene analysis. Strain SHE could utilize indole as the sole carbon source and almost completely degrade 100mg/L of indole within 24hr. It still harbored relatively high indole degradation capacity within pH4-9 and temperature 25°C-35°C. Experiments also showed that some heavy metals such as Mn(2+), Pb(2+) and Co(2+) did not pose severe inhibition on indole degradation. Based on high performance liquid chromatography-mass spectrum analysis, isatin was identified as a minor intermediate during the process of indole biodegradation. A major yellow product with m/z 265.0605 (C15H8N2O3) was generated and accumulated, suggesting a novel indole conversion pathway existed. Genome analysis of strain SHE indicated that there existed a rich set of oxidoreductases, which might be the key reason for the efficient degradation of indole. The robust degradation ability of strain SHE makes it a promising candidate for the treatment of indole containing wastewater.
吲哚是一种典型的含氮杂环芳香污染物，广泛存在于工业废水中。微生物降解已被证明是去除吲哚的一种可行方法，然而微生物资源相当有限。分离出一株名为SHE的细菌菌株，发现它是一种高效的吲哚降解菌。根据16S rRNA基因分析，它被鉴定为贪铜菌属。菌株SHE能够利用吲哚作为唯一碳源，并在24小时内几乎完全降解100mg/L的吲哚。在pH4-9和温度25°C-35°C范围内，它仍具有较高的吲哚降解能力。实验还表明，一些重金属如Mn(2+)、Pb(2+)和Co(2+)对吲哚降解没有严重抑制作用。基于高效液相色谱-质谱分析，异吲哚酮被确定为吲哚生物降解过程中的一种次要中间产物。产生并积累了一种主要的黄色产物，其m/z为265.0605（C15H8N2O3），表明存在一条新的吲哚转化途径。菌株SHE的基因组分析表明存在丰富的氧化还原酶，这可能是其高效降解吲哚的关键原因。菌株SHE强大的降解能力使其成为处理含吲哚废水的有前途的候选菌株。
