Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China.
Plant Cell Rep. 2012 Sep;31(9):1737-46. doi: 10.1007/s00299-012-1287-z. Epub 2012 May 30.
A novel orthologue of ethylene response factor gene, MsERF11, was isolated from alfalfa in this study. It has an open reading frame of 807 bp, encoding a predicted polypeptide of 268 amino acids. Sequence similarity analysis clearly suggested that MsERF11 encoded an ethylene response factor protein. The results of transient expression of MsERF11 in onion epidermal cells indicated that MsERF11 is a nuclear protein. The expression pattern of MsERF11 gene was analyzed by real-time quantitative PCR and a higher level of expression was observed in leaves than was observed in roots, stems, flower buds and flowers. Furthermore, the expression was induced by PEG6000, NaCl, Al2(SO4)3 and six different hormones. Over-expressing MsERF11 resulted in enhanced tolerances to salt stress in transgenic Arabidopsis plants. This research indicates that MsERF11 has the potential to be used for improving crop's salt tolerance in areas where salinity is a limiting factor for agricultural productivity.
MsERF11 was isolated from alfalfa. Its expression was induced by different abiotic stresses and hormones. Over-expressing MsERF11 resulted in enhanced salt tolerance in transgenic Arabidopsis plants.
本研究从紫花苜蓿中分离出乙烯反应因子基因的新直系同源物 MsERF11。它具有 807bp 的开放阅读框，编码 268 个氨基酸的预测多肽。序列相似性分析清楚地表明，MsERF11 编码乙烯反应因子蛋白。MsERF11 在洋葱表皮细胞中的瞬时表达结果表明，MsERF11 是一种核蛋白。通过实时定量 PCR 分析 MsERF11 基因的表达模式，发现其在叶片中的表达水平高于根、茎、花蕾和花。此外，它的表达还受到 PEG6000、NaCl、Al2(SO4)3 和六种不同激素的诱导。过表达 MsERF11 导致转基因拟南芥植物对盐胁迫的耐受性增强。这项研究表明，MsERF11 有可能被用于提高盐分限制农业生产力地区作物的耐盐性。
MsERF11 从紫花苜蓿中分离出来。它的表达受到不同非生物胁迫和激素的诱导。过表达 MsERF11 导致转基因拟南芥植物的耐盐性增强。
