State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
Collaborative Innovation Center, Jining Medical University, Jining, Shandong 272067, PR China.
Plant Cell Physiol. 2017 Oct 1;58(10):1673-1688. doi: 10.1093/pcp/pcx101.
High salinity is one of the most severe environmental stresses and limits the growth and yield of diverse crop plants. We isolated a gene named TaPUB1 from wheat (Triticum aestivum L. cv HF9703) that encodes a novel protein containing a U-box domain, the precursor RNA processing 19p (Prp19) superfamily and WD-40 repeats. Real-time reverse transcription-PCR analysis showed that TaPUB1 transcript accumulation was up-regulated by high salinity, drought and phytohormones, suggesting that it plays a role in the abiotic-related defense response. We overexpressed TaPUB1 in Nicotiana benthamiana to evaluate the function of TaPUB1 in the regulation of the salt stress response. Transgenic N. benthamiana plants (OE) with constitutively overexpressed TaPUB1 under the control of the Cauliflower mosaic virus 35S (CaMV 35S) promoter exhibited a higher germination rate, less growth inhibition, less Chl loss and higher photosynthetic capacity than wild-type (WT) plants under salt stress conditions. These results demonstrated the increased tolerance of OE plants to salt stress compared with the WT. The OE plants had lower osmotic potential (OP), reduced Na+ toxicity and less reactive oxygen species accumulation compared with the WT, which may be related to their higher level of osmolytes, lower Na+/K+ ratio and higher antioxidant enzyme activities under salt stress conditions. Consistent with these results, the up-regulated expression of osmic- and antioxidant-related genes in OE plants indicated a role for TaPUB1 in plant salt tolerance.
高盐度是最严重的环境胁迫之一，限制了各种作物的生长和产量。我们从小麦（Triticum aestivum L. cv HF9703）中分离出一个基因，命名为 TaPUB1，它编码一种含有 U -box 结构域、前体 RNA 加工 19p（Prp19）超家族和 WD-40 重复的新型蛋白质。实时反转录-PCR 分析表明，TaPUB1 转录物的积累受高盐度、干旱和植物激素的上调，表明它在非生物相关防御反应中发挥作用。我们在烟草（Nicotiana benthamiana）中过表达 TaPUB1，以评估 TaPUB1 在调节盐胁迫反应中的功能。在 Cauliflower mosaic virus 35S（CaMV 35S）启动子的控制下组成型过表达 TaPUB1 的转基因烟草（OE）植物在盐胁迫条件下表现出更高的发芽率、生长抑制减少、叶绿素损失减少和更高的光合能力，而野生型（WT）植物则表现出较低的发芽率、生长抑制增加、叶绿素损失增加和光合能力降低。这些结果表明，OE 植物对盐胁迫的耐受性比 WT 植物增加。OE 植物的渗透势（OP）较低，Na+毒性降低，活性氧积累减少，这可能与它们在盐胁迫条件下具有更高的渗透调节物质水平、更低的 Na+/K+比值和更高的抗氧化酶活性有关。与这些结果一致的是，OE 植物中与渗透和抗氧化相关的基因的上调表达表明 TaPUB1 在植物耐盐性中起作用。
