Peñalva Miguel A, Galindo Antonio, Abenza Juan F, Pinar Mario, Calcagno-Pizarelli Ana M, Arst Herbert N, Pantazopoulou Areti
Cell Logist. 2012 Jan 1;2(1):2-14. doi: 10.4161/cl.19304.
The genetically tractable filamentous ascomycete fungus Aspergillus nidulans has been successfully exploited to gain major insight into the eukaryotic cell cycle. More recently, its amenability to in vivo multidimensional microscopy has fueled a potentially gilded second age of A. nidulans cell biology studies. This review specifically deals with studies on intracellular membrane traffic in A. nidulans. The cellular logistics are subordinated to the needs imposed by the polarized mode of growth of the multinucleated hyphal tip cells, whereas membrane traffic is adapted to the large intracellular distances. Recent work illustrates the usefulness of this fungus for morphological and biochemical studies on endosome and Golgi maturation, and on the role of microtubule-dependent motors in the long-distance movement of endosomes. The fungus is ideally suited for genetic studies on the secretory pathway, as mutations impairing secretion reduce apical extension rates, resulting in phenotypes detectable by visual inspection of colonies.
遗传上易于处理的丝状子囊菌构巢曲霉已被成功用于深入了解真核细胞周期。最近，它对体内多维显微镜的适应性推动了构巢曲霉细胞生物学研究的第二个黄金时代。本综述专门讨论构巢曲霉细胞内膜运输的研究。细胞物流服从于多核菌丝尖端细胞极化生长模式所带来的需求，而膜运输则适应于较大的细胞内距离。最近的研究表明，这种真菌对于内体和高尔基体成熟的形态学和生物化学研究，以及微管依赖性马达在内体远距离移动中的作用研究很有用。这种真菌非常适合用于分泌途径的遗传学研究，因为损害分泌的突变会降低顶端延伸率，从而产生通过肉眼检查菌落即可检测到的表型。
