Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
J Am Chem Soc. 2012 Jun 20;134(24):9914-7. doi: 10.1021/ja303853y. Epub 2012 Jun 7.
A cell-specific metabolic glycan labeling strategy has been developed using azidosugars encapsulated in ligand-targeted liposomes. The ligands are designed to bind specific cell-surface receptors that are only expressed or up-regulated in target cells, which mediates the intracellular delivery of azidosugars. The delivered azidosugars are metabolically incorporated into cell-surface glycans, which are then imaged via a bioorthogonal reaction.
一种细胞特异性代谢聚糖标记策略已经开发出来，该策略使用包封在配体靶向脂质体中的叠氮糖。这些配体被设计为与仅在靶细胞中表达或上调的特定细胞表面受体结合，从而介导叠氮糖的细胞内递送。递送到细胞内的叠氮糖被代谢掺入到细胞表面聚糖中，然后通过生物正交反应进行成像。
