Infection Biology Research Program, Department of Virology, Haartman Institute, Faculty of Medicine, PO Box 21, FI-00014 University of Helsinki, Helsinki, Finland.
Department of Virology and Immunology, HUSLAB, PO Box 400, FI-00029 HUS, Helsinki, Finland.
J Gen Virol. 2012 Sep;93(Pt 9):1984-1990. doi: 10.1099/vir.0.042853-0. Epub 2012 May 30.
Sindbis virus (SINV) is an arthropod-borne alphavirus, which causes rash-arthritis, particularly in Finland. SINV is transmitted by mosquitoes in Finland but thus far no virus has been isolated from mosquitoes. In this study, we report the isolation of the first SINV strain from mosquitoes in Finland and its full-length protein-coding sequence. We furthermore describe the full-length coding sequence of six SINV strains previously isolated from humans in Finland and from a mosquito in Russia. The strain isolated from mosquitoes (Ilomantsi-2005M) was very closely related to all the other Northern European SINV strains. We found 9 aa positions, of which five in the nsP3 protein C terminus, to be distinctive signatures for the Northern European strains that may be associated with vector or host species adaptation. Phylogenetic analyses further indicate that SINV has a local circulation in endemic regions in Northern Europe and no novel strains are frequently being introduced.
辛德比斯病毒（SINV）是一种节肢动物传播的甲病毒，可引起皮疹-关节炎，尤其在芬兰。芬兰通过蚊子传播 SINV，但迄今为止尚未从蚊子中分离出病毒。在这项研究中，我们报告了从芬兰蚊子中分离出的首例 SINV 株及其全长蛋白编码序列。此外，我们还描述了先前从芬兰人和俄罗斯蚊子中分离出的六种 SINV 株的全长编码序列。从蚊子中分离出的菌株（Ilomantsi-2005M）与所有其他北欧 SINV 株非常密切相关。我们发现 9 个 aa 位置，其中 5 个位于 nsP3 蛋白 C 端，是北欧株的独特特征，可能与媒介或宿主物种的适应性有关。系统发育分析进一步表明，SINV 在北欧地方性流行地区有局部循环，没有新的流行株频繁传入。
