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九种芬兰物种的 RNA 病毒组特征。

Characterisation of the RNA Virome of Nine Species in Finland.

机构信息

Department of Virology, Medicum, University of Helsinki, Haartmaninkatu 3, FI-00290 Helsinki, Finland.

The Natural History Museum, Cromwell Road, South Kensington, London SW5 7BD, UK.

出版信息

Viruses. 2022 Jul 7;14(7):1489. doi: 10.3390/v14071489.

DOI:10.3390/v14071489
PMID:35891469
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9324324/
Abstract

RNA viromes of nine commonly encountered mosquito species collected around Finland in 2015 and 2017 were studied using next-generation sequencing. Mosquito homogenates were sequenced from 91 pools comprising 16-60 morphologically identified adult females of , , , , , , , and /. In total 514 viral Reverse dependent RNA polymerase (RdRp) sequences of 159 virus species were recovered, belonging to 25 families or equivalent rank, as follows: , , , , , , , , , , , , , , , , , , , , , , , and . Of these, 147 are tentatively novel viruses. One sequence of , which causes Pogosta disease in humans, was detected from from Pohjois-Karjala. This study greatly increases the number of mosquito-associated viruses known from Finland and presents the northern-most mosquito-associated viruses in Europe to date.

摘要

利用下一代测序技术研究了 2015 年和 2017 年在芬兰周边地区采集的 9 种常见蚊子的 RNA 病毒组。从 91 个由 16-60 只形态鉴定的成年雌性蚊子组成的混合样本中对蚊子进行了测序,这些蚊子分别属于 、 、 、 、 、 、 、 和 。共回收了 514 个属于 25 个科或同等分类等级的 159 种病毒的 RdRp 序列,分别为 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 和 。其中 147 个为暂定的新型病毒。从 Pohjois-Karjala 的 中检测到一种导致 Pogosta 病的病毒的序列。本研究极大地增加了芬兰已知的蚊子相关病毒数量,并展示了迄今为止欧洲最北部的蚊子相关病毒。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5e6/9324324/d074f1b0c70a/viruses-14-01489-g018.jpg
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