Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha, China.
Acta Biochim Biophys Sin (Shanghai). 2012 Jul;44(7):584-90. doi: 10.1093/abbs/gms042. Epub 2012 May 30.
Nasopharyngeal carcinoma-associated gene 6 (NGX6; syn. transmembrane protein 8B, TMEM8B) is a recently identified tumor suppressor gene. The underlying mechanisms by which the gene inhibits tumor development are not completely known. To further understand the function of the gene's protein product NGX6, in the present study, we employed two-dimensional difference gel electrophoresis to analyze the protein expression profiles of colon cancer HT-29 cells stably transfected with the gene NGX6. The differentially expressed proteins were selected and identified by matrix-assisted laser desorption/ionization coupled with time-of-flight tandem mass spectrometry. The results showed that 12 proteins were down-regulated and 4 were up-regulated in NGX6-transfected HT-29 cells, compared with vector-transfected HT-29 cells. The MS results were verified by western blot. Bioinformatic analysis showed that these proteins are involved in cell proliferation, metastasis, apoptosis, cytoskeletal structure, metabolism, and signal transduction, suggesting that NGX6 may inhibit colon cancer through the regulation of these biological processes.
鼻咽癌相关基因 6（NGX6；同义词：跨膜蛋白 8B，TMEM8B）是最近发现的一种肿瘤抑制基因。该基因抑制肿瘤发生的潜在机制尚不完全清楚。为了进一步了解基因蛋白产物 NGX6 的功能，本研究采用二维差异凝胶电泳分析了转染 NGX6 基因的结肠癌 HT-29 细胞的蛋白质表达谱。通过基质辅助激光解吸/电离与飞行时间串联质谱联用技术选择和鉴定差异表达的蛋白质。结果显示，与空载体转染的 HT-29 细胞相比，NGX6 转染的 HT-29 细胞中有 12 种蛋白下调，4 种蛋白上调。MS 结果经 Western blot 验证。生物信息学分析表明，这些蛋白参与细胞增殖、转移、凋亡、细胞骨架结构、代谢和信号转导，提示 NGX6 可能通过调节这些生物学过程抑制结肠癌。
