Ketelsen A T, Johnson D W, Muscoplat C C
Infect Immun. 1979 Aug;25(2):565-68. doi: 10.1128/iai.25.2.565-568.1979.
Incubation of bovine peripheral blood monocytes with bovine viral diarrhea virus (either Singer or NY-1 strain) caused a consistent, statistically significant decrease in their random locomotion (no chemoattractant) and chemotaxis towards a chemotactic lymphokine. Chemotaxis was determined by a modification of the Boyden method. Incubation of bovine viral diarrhea virus with mononuclear cells depressed chemotaxis by a mean of 56% (P less than 0.0005). Heat-killed virus had no effect on monocyte motility. Data suggest that bovine viral diarrhea virus can rapidly suppress monocyte functions in vitro, but by unknown mechanisms, not by killing cells.
用牛病毒性腹泻病毒（辛格尔毒株或NY - 1毒株）孵育牛外周血单核细胞，会导致其随机运动（无趋化因子）以及对趋化性淋巴因子的趋化作用出现持续且具有统计学显著意义的降低。趋化性通过改良的博伊登方法测定。牛病毒性腹泻病毒与单核细胞孵育后，趋化作用平均降低了56%（P小于0.0005）。热灭活病毒对单核细胞运动没有影响。数据表明，牛病毒性腹泻病毒在体外可迅速抑制单核细胞功能，但机制不明，并非通过杀死细胞来实现。
