Department of Animal Science, Colorado State University, Fort Collins, CO, USA.
J Anim Sci. 2012 Jun;90(6):1948-54. doi: 10.2527/jas.2011-4059.
Thirty-three colostrum-deprived Holstein bull calves (initial BW of 131 ± 4 kg) were used to determine the effect of timing of anthelmintic administration relative to vaccination on antibody titer response to vaccine component antigens. When calves were at least 3 mo of age, they were sorted randomly into individual pens and assigned to 1 of 3 treatment groups, treatments consisted of 1) dewormed 2 wk before vaccination (DPV), 2) dewormed at the time of vaccination (DV), or 3) control, vaccinated but not dewormed (CONT). All calves were inoculated with infective larvae of brown stomach worms (Ostertagia ostertagi) and intestinal worms (Cooperia spp.) on d 1, 7, 10, 14, and 18 for a total dose of 235,710 infective larvae per calf. Calves (DPV and DV) were dewormed on d 21 or 35 with a 10% fenbendazole suspension at 5 mg/kg of BW. On d 35, all calves were vaccinated with a modified-live virus respiratory vaccine containing IBRV (infectious bovine rhinotracheitis virus), BVDV-1 (bovine viral diarrhea virus genotype 1), BVDV-2 (BVDV genotype 2), PI-3 (parainfluenza-3), and BRSV (bovine respiratory syncytial virus). During the 103-d experiment, weekly fecal egg counts, blood, and rectal temperatures were collected and health status was recorded daily. Blood samples were obtained weekly to determine serum neutralizing (SN) antibody titers to IBRV, BVDV-1, BVDV-2, and PI-3 and cytokine levels for IL-4, IL-6, TNF-α (tumor necrosis factor-α), and IFN-γ (interferon-gamma). There was a tendency (P < 0.09) for CONT calves to have greater IL-4 concentrations. By design, control calves had greater (P < 0.01) fecal egg counts during the experiment. All calves developed antibody titers to IBRV, BVDV-1, BVDV-2, and PI-3 by d 15 postvaccination. On d 88, all calves were challenged with IBRV and blood samples were obtained on d 88, 89, 90, 93, 95, 98, 99, and 103. All calves had increased rectal temperatures during the final 7 d of the IBRV challenge. However, the CONT group had greater (P < 0.01) rectal temperatures on each sampling day except d 90 compared with the DPV and DV treatments. Therefore, deworming before or at vaccination reduced parasite burden and decreased rectal temperature increase after an IBRV challenge. Deworming strategy had no effect on antibody response to vaccination or IBRV challenge.
33 头初乳不足的荷斯坦公牛犊（初始体重 131±4kg）被用于确定驱虫药的使用时间相对疫苗接种对疫苗成分抗原抗体滴度反应的影响。当犊牛至少 3 月龄时，将其随机分成单独的围栏，并分配到 3 个处理组中的 1 个，处理组包括 1）驱虫 2 周前（DPV），2）接种时驱虫（DV），或 3）对照，接种但不驱虫（CONT）。所有犊牛在第 1、7、10、14 和 18 天接种棕色胃线虫（Ostertagia ostertagi）和肠道线虫（Cooperia spp.）的感染性幼虫，每头犊牛共接种 235710 条感染性幼虫。犊牛（DPV 和 DV）于第 21 天或第 35 天用 10%芬苯达唑混悬液按 5mg/kg BW 驱虫。第 35 天，所有犊牛均用含有 IBRV（传染性牛鼻气管炎病毒）、BVDV-1（牛病毒性腹泻病毒 1 型）、BVDV-2（BVDV 2 型）、PI-3（副流感-3）和 BRSV（牛呼吸道合胞体病毒）的改良活病毒呼吸道疫苗接种。在 103 天的实验期间，每周收集粪便卵、血液和直肠温度，并记录健康状况。每周采集血液样本以确定 IBRV、BVDV-1、BVDV-2 和 PI-3 的血清中和（SN）抗体滴度，并检测 IL-4、IL-6、TNF-α（肿瘤坏死因子-α）和 IFN-γ（干扰素-γ）的细胞因子水平。CONT 犊牛的 IL-4 浓度有升高趋势（P<0.09）。通过设计，对照犊牛在实验期间的粪便卵计数更高（P<0.01）。所有犊牛在接种后第 15 天均产生 IBRV、BVDV-1、BVDV-2 和 PI-3 的抗体滴度。第 88 天，所有犊牛均用 IBRV 攻毒，于第 88、89、90、93、95、98、99 和 103 天采集血液样本。所有犊牛在 IBRV 攻毒的最后 7 天内直肠温度升高。然而，CONT 组在每个采样日的直肠温度均高于 DPV 和 DV 组，除第 90 天外，差异均有统计学意义（P<0.01）。因此，在接种前或同时驱虫可降低寄生虫负荷，并降低 IBRV 攻毒后的直肠温度升高。驱虫策略对疫苗接种或 IBRV 攻毒的抗体反应没有影响。
