Leterrier Marina, Barroso Juan B, Valderrama Raquel, Palma José M, Corpas Francisco J
Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080 Granada, Spain.
ScientificWorldJournal. 2012;2012:694740. doi: 10.1100/2012/694740. Epub 2012 May 2.
NADPH regeneration appears to be essential in the mechanism of plant defence against oxidative stress. Plants contain several NADPH-generating dehydrogenases including isocitrate dehydrogenase (NADP-ICDH), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), and malic enzyme (ME). In Arabidopsis seedlings grown under salinity conditions (100 mM NaCl) the analysis of physiological parameters, antioxidant enzymes (catalase and superoxide dismutase) and content of superoxide radical (O2∙-), nitric oxide (NO), and peroxynitrite (ONOO(-)) indicates a process of nitro-oxidative stress induced by NaCl. Among the analysed NADPH-generating dehydrogenases under salinity conditions, the NADP-ICDH showed the maximum activity mainly attributable to the root NADP-ICDH. Thus, these data provide new insights on the relevance of the NADP-ICDH which could be considered as a second barrier in the mechanism of response against the nitro-oxidative stress generated by salinity.
NADPH再生在植物抵御氧化应激的机制中似乎至关重要。植物含有几种产生NADPH的脱氢酶,包括异柠檬酸脱氢酶(NADP-ICDH)、葡萄糖-6-磷酸脱氢酶(G6PDH)、6-磷酸葡萄糖酸脱氢酶(6PGDH)和苹果酸酶(ME)。在盐胁迫条件(100 mM NaCl)下生长的拟南芥幼苗中,对生理参数、抗氧化酶(过氧化氢酶和超氧化物歧化酶)以及超氧阴离子(O2∙-)、一氧化氮(NO)和过氧亚硝酸根(ONOO(-))含量的分析表明,NaCl诱导了硝基氧化应激过程。在盐胁迫条件下分析的产生NADPH的脱氢酶中,NADP-ICDH表现出最大活性,主要归因于根部的NADP-ICDH。因此,这些数据为NADP-ICDH的相关性提供了新的见解,NADP-ICDH可被视为应对盐胁迫产生的硝基氧化应激机制中的第二道防线。