Zhang Dongyang, Wang Di, Xu Ning, Feng Siyu, Qian Ying, Wang Shuheng, Bai Yun, Zhou Yunwei
College of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin, 130118, China.
Tonghua Normal University, No. 950 Yu Cai Road, Dongchang District, Tonghua City, 134002, China.
BMC Plant Biol. 2025 Mar 19;25(1):357. doi: 10.1186/s12870-025-06384-2.
Saline-alkali stress is one of the main abiotic stresses that constrains plant growth. Understanding the response mechanism of ornamental plants to saline-alkali stress is of great significance for improving saline-alkali landscape greening. Chrysanthemum is a good ornamental plant with strong resistance to stress, rich colors and easy management.
Using TMT quantitative proteomics technology, leave and root of Chrysanthemum that were either untreated or treated with 200 mM NaCl for 12 h, screened the differentially expressed proteins. The results showed that 66 and 452 differential proteins were present in leaves and roots after salt treatment, respectively. GO function is mainly related to carbohydrate and energy metabolism, hormone response, antioxidant response and membrane protein activity. The KEGG metabolic pathway is mainly concentrated in glycine metabolism, glutathione metabolic pathway, carbon fixation in prokaryotes, 2-oxy-carboxylic acid metabolism. Combining transcripto-proteomics, GO and KEGG analyses revealed significant enrichment in starch anabolic catabolism, redox processes, ion homeostatic transport, phenylpropane biosynthesis.
Under salt stress, the active pathways of carbohydrate and energy metabolism and glutathione metabolism enable plants to accumulate more energy substances and improve antioxidant capacity, which may play a safeguarding role in maintaining growth and development and mitigating reactive oxygen species damage in Chrysanthemum under stress. The purpose of this study was to screen key proteins and regulatory networks through proteomic assay, and reveal the molecular mechanism of response to salt stress. The research not only provides resources for salt-tolerant breeding of Chrysanthemum but also offers theoretical support for agricultural production and ecological environmental protection.
盐碱胁迫是制约植物生长的主要非生物胁迫之一。了解观赏植物对盐碱胁迫的响应机制对于改善盐碱地景观绿化具有重要意义。菊花是一种优良的观赏植物，抗逆性强，花色丰富，易于管理。
采用TMT定量蛋白质组学技术，对未处理及用200 mM NaCl处理12 h的菊花叶片和根系进行差异表达蛋白筛选。结果表明，盐处理后叶片和根系分别有66个和452个差异蛋白。GO功能主要与碳水化合物和能量代谢、激素响应、抗氧化响应及膜蛋白活性相关。KEGG代谢途径主要集中在甘氨酸代谢、谷胱甘肽代谢途径、原核生物中的碳固定、2-氧代羧酸代谢。结合转录组-蛋白质组学、GO和KEGG分析发现，淀粉合成分解代谢、氧化还原过程、离子稳态转运、苯丙烷生物合成显著富集。
在盐胁迫下，碳水化合物和能量代谢以及谷胱甘肽代谢的活跃途径使植物能够积累更多的能量物质并提高抗氧化能力，这可能在维持菊花在胁迫下的生长发育和减轻活性氧损伤方面发挥保护作用。本研究旨在通过蛋白质组学分析筛选关键蛋白和调控网络，揭示菊花对盐胁迫的响应分子机制。该研究不仅为菊花耐盐育种提供资源，也为农业生产和生态环境保护提供理论支持。
