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经典肠出血性大肠杆菌血清型和德国大肠杆菌 O104:H4 暴发株志贺毒素 2 噬菌体的比较。

A comparison of Shiga-toxin 2 bacteriophage from classical enterohemorrhagic Escherichia coli serotypes and the German E. coli O104:H4 outbreak strain.

机构信息

Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta, Canada.

出版信息

PLoS One. 2012;7(5):e37362. doi: 10.1371/journal.pone.0037362. Epub 2012 May 23.

Abstract

Escherichia coli O104:H4 was associated with a severe foodborne disease outbreak originating in Germany in May 2011. More than 4000 illnesses and 50 deaths were reported. The outbreak strain was a typical enteroaggregative E. coli (EAEC) that acquired an antibiotic resistance plasmid and a Shiga-toxin 2 (Stx2)-encoding bacteriophage. Based on whole-genome phylogenies, the O104:H4 strain was most closely related to other EAEC strains; however, Stx2-bacteriophage are mobile, and do not necessarily share an evolutionary history with their bacterial host. In this study, we analyzed Stx2-bacteriophage from the E. coli O104:H4 outbreak isolates and compared them to all available Stx2-bacteriophage sequences. We also compared Stx2 production by an E. coli O104:H4 outbreak-associated isolate (ON-2011) to that of E. coli O157:H7 strains EDL933 and Sakai. Among the E. coli Stx2-phage sequences studied, that from O111:H- strain JB1-95 was most closely related phylogenetically to the Stx2-phage from the O104:H4 outbreak isolates. The phylogeny of most other Stx2-phage was largely concordant with their bacterial host genomes. Finally, O104:H4 strain ON-2011 produced less Stx2 than E. coli O157:H7 strains EDL933 and Sakai in culture; however, when mitomycin C was added, ON-2011 produced significantly more toxin than the E. coli O157:H7 strains. The Stx2-phage from the E. coli O104:H4 outbreak strain and the Stx2-phage from O111:H- strain JB1-95 likely share a common ancestor. Incongruence between the phylogenies of the Stx2-phage and their host genomes suggest the recent Stx2-phage acquisition by E. coli O104:H4. The increase in Stx2-production by ON-2011 following mitomycin C treatment may or may not be related to the high rates of hemolytic uremic syndrome associated with the German outbreak strain. Further studies are required to determine whether the elevated Stx2-production levels are due to bacteriophage or E. coli O104:H4 host related factors.

摘要

产志贺毒素 2 型(Stx2)大肠埃希菌 O104:H4 于 2011 年 5 月在德国引发了一起严重的食源性疾病暴发。超过 4000 人患病,50 人死亡。暴发菌株是一种典型的肠聚集性大肠埃希菌(EAEC),获得了一个抗生素抗性质粒和一个编码志贺毒素 2 型(Stx2)的噬菌体。基于全基因组系统发育分析,O104:H4 菌株与其他 EAEC 菌株最为密切相关;然而,Stx2 噬菌体是可移动的,并不一定与其细菌宿主具有共同的进化史。在这项研究中,我们分析了来自大肠杆菌 O104:H4 暴发分离株的 Stx2 噬菌体,并将其与所有可用的 Stx2 噬菌体序列进行了比较。我们还比较了与大肠杆菌 O104:H4 暴发相关的分离株(ON-2011)与大肠杆菌 O157:H7 菌株 EDL933 和 Sakai 之间的 Stx2 产生情况。在所研究的大肠杆菌 Stx2 噬菌体序列中,O111:H- 菌株 JB1-95 的噬菌体在系统发育上与 O104:H4 暴发分离株的噬菌体最为密切相关。大多数其他 Stx2 噬菌体的系统发育与它们的细菌宿主基因组基本一致。最后,大肠杆菌 O104:H4 菌株 ON-2011 在培养物中产生的 Stx2 少于大肠杆菌 O157:H7 菌株 EDL933 和 Sakai;然而,当加入丝裂霉素 C 时,ON-2011 产生的毒素明显多于大肠杆菌 O157:H7 菌株。大肠杆菌 O104:H4 暴发株的 Stx2 噬菌体和 O111:H- 菌株 JB1-95 的 Stx2 噬菌体可能有共同的祖先。Stx2 噬菌体与其宿主基因组之间的系统发育不一致表明,大肠杆菌 O104:H4 最近获得了 Stx2 噬菌体。ON-2011 在丝裂霉素 C 处理后 Stx2 产量的增加可能与德国暴发菌株相关的溶血性尿毒综合征的高发病率有关,也可能无关。需要进一步研究以确定 Stx2 产量水平升高是否归因于噬菌体或大肠杆菌 O104:H4 宿主相关因素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a84/3359367/38efd93ee268/pone.0037362.g001.jpg

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