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用表达流感 H5 病毒血凝素的重组腺病毒载体诱导针对流感病毒的保护性异源免疫应答。

Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus.

机构信息

Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences.

出版信息

Acta Naturae. 2010 Apr;2(1):111-8.

PMID:22649637
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3347542/
Abstract

Influenza viruses are characterized by a high degree of antigenic variability, which causes the annual emergence of flu epidemics and irregularly timed pandemics caused by viruses with new antigenic and biological traits. Novel approaches to vaccination can help circumvent this problem. One of these new methods incorporates genetic vaccines based on adenoviral vectors. Recombinant adenoviral vectors which contain hemagglutinin-encoding genes from avian H5N1 and H5N2 (Ad-HA5-1 and Ad-HA5-2) influenza viruses were obtained using the AdEasy Adenoviral Vector System (Stratagene). Laboratory mice received a double intranasal vaccination with Ad-HA5-1 and Ad-HA5-2. This study demonstrates that immunization with recombinant adenoviruses bearing the Н 5 influenza virus hemagglutinin gene induces a immune response which protects immunized mice from a lethal dose of the H5 influenza virus. Moreover, it also protects the host from a lethal dose of the H1 virus, which belongs to the same clade as H5, but does not confer protection from the subtype H3 influenza virus, which belongs to a different clade.

摘要

流感病毒的一个显著特点是抗原高度可变，这导致每年都会出现流感疫情，并会不时出现具有新抗原和生物学特征的病毒引发的大流行。新型疫苗接种方法可以帮助解决这个问题。其中一种新方法是使用基于腺病毒载体的基因疫苗。通过 AdEasy 腺病毒载体系统（Stratagene）获得了含有禽流感 H5N1 和 H5N2（Ad-HA5-1 和 Ad-HA5-2）流感病毒血凝素编码基因的重组腺病毒载体。实验室小鼠接受了两次鼻腔内接种 Ad-HA5-1 和 Ad-HA5-2 的双重免疫。本研究表明，接种携带 H5 流感病毒血凝素基因的重组腺病毒可诱导免疫反应，使免疫小鼠免受 H5 流感病毒致死剂量的侵害。此外，它还可以保护宿主免受同属一个分支的 H1 病毒的致死剂量的侵害，但不能预防属于不同分支的 H3 流感病毒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73a1/3347542/611a66e52f5e/AN20758251-04-111-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73a1/3347542/611a66e52f5e/AN20758251-04-111-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73a1/3347542/72674188547b/AN20758251-04-111-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73a1/3347542/6c1cf76f9077/AN20758251-04-111-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73a1/3347542/185047b32583/AN20758251-04-111-g003.jpg
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