Gamaleya Research Institute of Epidemiology and Microbiology, Gamaleya Str., 18, Moscow, Russia, 123098.
Acta Naturae. 2012 Oct;4(4):17-27.
This review covers the problems encountered in the construction and production of new recombinant influenza vaccines. New approaches to the development of influenza vaccines are investigated; they include reverse genetics methods, production of virus-like particles, and DNA- and viral vector-based vaccines. Such approaches as the delivery of foreign genes by DNA- and viral vector-based vaccines can preserve the native structure of antigens. Adenoviral vectors are a promising gene-delivery platform for a variety of genetic vaccines. Adenoviruses can efficiently penetrate the human organism through mucosal epithelium, thus providing long-term antigen persistence and induction of the innate immune response. This review provides an overview of the practicability of the production of new recombinant influenza cross-protective vaccines on the basis of adenoviral vectors expressing hemagglutinin genes of different influenza strains.
本文综述了新型重组流感疫苗研制和生产中遇到的问题。对新型流感疫苗的开发方法进行了研究，包括反向遗传学方法、病毒样颗粒的生产以及基于 DNA 和病毒载体的疫苗。基于 DNA 和病毒载体的疫苗传递外源基因等方法可以保持抗原的天然结构。腺病毒载体是多种基因疫苗的有前途的基因传递平台。腺病毒可以通过黏膜上皮有效地穿透人体，从而提供长期的抗原持久性和诱导先天免疫反应。本文综述了基于表达不同流感株血凝素基因的腺病毒载体生产新型重组流感交叉保护疫苗的实用性。
