Division of Immunogenetics, German Cancer Center, Heidelberg, Germany.
Acta Naturae. 2010 Jul;2(2):96-102.
Stimulation of CD95 (APO-1/Fas) leads to apoptosis induction in multicellular organisms. CD95-mediated apoptosis starts with the formation of the protein complex at the receptor CD95 (APO-1/Fas), which was named DISC (death-inducing signaling complex). In this work, the composition of the CD95 DISC in two different cell types was analyzed using proteomics approaches. Using 2D gels, the composition of the CD95 DISC was analyzed in the so-called Type I and Type II cells, which are characterized by different kinetics of apoptosis. The detailed analysis of the CD95 DISC performed by 2D gels demonstrated that, besides the well-established components of the CD95 DISC, which are present in both cell types (CD95, FADD and procaspase-8), there are a number of differential spots detected at the CD95 DISC of Type I versus Type II cells. Taken together, this work demonstrates the differential composition of the CD95 DISC of Type I versus Type II cells.
CD95（APO-1/Fas）的刺激导致多细胞生物中的细胞凋亡诱导。CD95 介导的细胞凋亡始于受体 CD95（APO-1/Fas）上的蛋白质复合物的形成，该复合物被命名为 DISC（死亡诱导信号复合物）。在这项工作中，使用蛋白质组学方法分析了两种不同细胞类型中 CD95 DISC 的组成。使用 2D 凝胶，分析了所谓的 I 型和 II 型细胞中 CD95 DISC 的组成，这两种细胞的凋亡动力学不同。通过 2D 凝胶对 CD95 DISC 的详细分析表明，除了存在于两种细胞类型中的 CD95 DISC 的既定组成部分（CD95、FADD 和 procaspase-8）外，在 I 型与 II 型细胞的 CD95 DISC 中还检测到许多差异点。总之，这项工作证明了 I 型与 II 型细胞的 CD95 DISC 的差异组成。
