Biology Department, Lomonosov Moscow State University.
Acta Naturae. 2011 Jul;3(3):40-6.
Potato virus X (PVX) and some other potexviruses can be reconstitutedin vitrofrom viral coat protein (CP) and RNA. PVX CP is capable of forming viral ribonucleoprotein complexes (vRNP) not only with homologous, but also with foreign RNAs. This paper presents the structure and properties of vRNP assembledin vitroupon incubation of PVX CP and RNAs of various plant and animal viruses belonging to different taxonomic groups. We have shown that the morphology and translational properties of vRNPs containing foreign (heterologous) RNA are identical to those of homological vRNP (PVX RNA - PVX CP). Our data suggest that the assembly of the "mixed" vRNPin vitrocould be started at the 5'-proximal region of the RNA, producing a helical structure of vRNPs with foreign nucleic acids. The formation of heterologous vRNPin vitrowith PVX CP appears not to require a specific 5' end RNA nucleotide sequence, and the PVX CP seems to be able to pack foreign genetic material of various sizes and compositions into artificial virus-like particles.
马铃薯 X 病毒（PVX）和其他一些马铃薯病毒在体外可以由病毒外壳蛋白（CP）和 RNA 重新组成。PVX CP 不仅可以与同源 RNA，还可以与异源 RNA 形成病毒核糖核蛋白复合物（vRNP）。本文介绍了在体外孵育 PVX CP 和属于不同分类群的各种植物和动物病毒的 RNA 时组装的 vRNP 的结构和特性。我们已经表明，含有异源（异源）RNA 的 vRNP 的形态和翻译性质与同源 vRNP（PVX RNA-PVX CP）相同。我们的数据表明，“混合”vRNP 在体外的组装可以从 RNA 的 5' 近端区域开始，产生具有异源核酸的 vRNP 的螺旋结构。用 PVX CP 在体外形成异源 vRNP 似乎不需要特定的 5' 端 RNA 核苷酸序列，并且 PVX CP 似乎能够将各种大小和组成的异源遗传物质包装成人工病毒样颗粒。
