Indyk Harvey, Dowell Dawn
Fonterra Co-operative Group Ltd, Waitoa, New Zealand.
J AOAC Int. 2012 Mar-Apr;95(2):298-300.
After a review of data from a single-laboratory validation (SLV) study published in the International Dairy Journal 21, 783-789 (2011), a method for folate in infant formula and adult/pediatric nutritional formula was submitted for consideration of adoption by AOAC as an automated assay that is rapid and simple. The method uses an optical biosensor assay to quantitate total folate content in milk and milk-based pediatric and adult nutritional products. The assay uses folate binding protein and a functionalized sensor surface. The SLV showed an instrumental LOD of 0.1 ng/mL (equivalent to 2.5 microg/100 g for a typical infant formula). The method detection limit was 6.5 microg/100 g with a repeatability of 3.48% and an intermediate reproducibility of 4.63% RSD.
在对发表于《国际乳品杂志》21卷，783 - 789页（2011年）的单实验室验证（SLV）研究数据进行审查之后，一种用于婴儿配方奶粉和成人/儿童营养配方奶粉中叶酸的测定方法被提交给AOAC，以供其考虑采用为一种快速且简便的自动化分析方法。该方法使用光学生物传感器分析来定量牛奶以及以牛奶为基础的儿童和成人营养产品中的总叶酸含量。该分析使用叶酸结合蛋白和功能化的传感器表面。单实验室验证显示仪器检测限为0.1 ng/mL（相当于典型婴儿配方奶粉中2.5 μg/100 g）。该方法的检测限为6.5 μg/100 g，重复性为3.48%，中间精密度相对标准偏差（RSD）为4.63%。
