DeVries Jonathan W, Silvera Karlene R, McSherry Elliot, Dowell Dawn
Medallion Laboratories, 9000 Plymouth Ave North, Minneapolis, MN 55427, USA.
J AOAC Int. 2012 Mar-Apr;95(2):322-8. doi: 10.5740/jaoacint.cs2011_15.
During the "Standards Development and International Harmonization: AOAC INTERNATIONAL Mid-Year Meeting," held on June 29, 2011, an Expert Review Panel (ERP) reviewed the method for the "Determination of Vitamins A (Retinol) and E (alpha-Tocopherol) in Foods by Liquid Chromatography: Collaborative Study," published by Jonathan W. DeVries and Karlene R. Silvera in J. AOAC Int. in 2002. After evaluation of the original validation data, an ERP agreed in June 2011 that the method meets standard method performance requirements (SMPRs) for vitamin A, as articulated by the Stakeholder Panel on Infant Formula and Adult Nutritionals. The ERP granted the method First Action status, applicable to determining vitamin A in ready-to-eat infant and adult nutritional formula. In an effort to achieve Final Action status, it was recommended that additional information be generated for different types of infant and adult nutritional formula matrixes at varied concentration levels as indicated in the vitamin A (retinol) SMPR. Existing AOAC LC methods are suited for specific vitamin A analytical applications. The original method differs from existing methods in that it can be used to assay samples in all nine sectors of the food matrix. One sector of the food matrix was powdered infant formula and gave support for the First Action approval for vitamin A in infant and adult nutritional formula. In this method, standards and test samples are saponified in basic ethanol-water solution, neutralized, and diluted, converting fats to fatty acids and retinol esters to retinol. Retinol is quantitated by an LC method, using UV detection at 313 or 328 nm for retinol. Vitamin concentration is calculated by comparison of the peak heights or peak areas of retinol in test samples with those of standards.
在2011年6月29日举行的“标准制定与国际协调：AOAC国际年中会议”上，一个专家评审小组（ERP）审查了乔纳森·W·德弗里斯和卡琳·R·西尔韦拉于2002年发表在《美国分析化学家协会杂志》上的“液相色谱法测定食品中的维生素A（视黄醇）和维生素E（α-生育酚）：协作研究”方法。在评估原始验证数据后，ERP于2011年6月认定该方法符合婴儿配方食品和成人营养产品利益相关者小组所阐述的维生素A标准方法性能要求（SMPR）。ERP授予该方法首次行动状态，适用于即食婴儿和成人营养配方食品中维生素A的测定。为了获得最终行动状态，建议按照维生素A（视黄醇）SMPR的要求，针对不同类型、不同浓度水平的婴儿和成人营养配方食品基质生成更多信息。现有的AOAC液相色谱法适用于特定的维生素A分析应用。原始方法与现有方法的不同之处在于，它可用于分析食品基质所有九个部分的样品。食品基质的一个部分是婴儿配方奶粉，这为婴儿和成人营养配方食品中维生素A的首次行动批准提供了支持。在此方法中，标准品和测试样品在碱性乙醇 - 水溶液中皂化，中和并稀释，将脂肪转化为脂肪酸，视黄醇酯转化为视黄醇。视黄醇通过液相色谱法进行定量，使用313或328nm的紫外检测来测定视黄醇。通过比较测试样品中视黄醇的峰高或峰面积与标准品的峰高或峰面积来计算维生素浓度。
