Campos-Giménez Esther, Fontannaz Patric, Trisconi Marie-José, Kilinc Tamara, Gimenez Catherine, Andrieux Pierre, Nelson Maria
Nestlé Research Center, Vers-chez-les-blanc, CH-1000 Lausanne 26, Switzerland.
J AOAC Int. 2012 Mar-Apr;95(2):307-12. doi: 10.5740/jaoacint.cs2011_08.
At the "Standards Development and International Harmonization: AOAC INTERNATIONAL Mid-Year Meeting," on June 29, 2011, an Expert Review Panel agreed that the method "Determination of Vitamin B12 in Infant Formulas and Adult Nutritionals by Liquid Chromatography/UV Detection with Immunoaffinity Extraction" be adopted AOAC Official First Action status. The method is applicable for the determination of vitamin B12, which includes added cyanocobalamin and natural forms, making it applicable to both fortified and nonfortified products. Vitamin B12 is extracted from the sample in sodium acetate buffer in the presence of sodium cyanide (100 degrees C, 30 min). After purification and concentration with an immunoaffinity column, vitamin B12 is determined by LC with UV detection (361 nm). A single-laboratory validation study was conducted on a range of products, including milk- and soy-based infant formulas, cereals, cocoa beverages, health care products, and polyvitamin premixes. The method demonstrated linear response over a large range of concentrations, recovery rates of 100.8 +/- 7.5% (average +/- SD), repeatability RSD (RSDr) of 2.1%, and intermediate reproducibility (RSD(iR)) of 4.3%. LOD and LOQ values were 0.10 and 0.30 microg/100 g, respectively, and correlation with the reference microbiological assay was good (R2 = 0.9442). The results of the study were published in J. AOAC Int. 91, 786-793 (2008). The performance characteristics of the method met the standard method performance requirements set forth by the Stakeholder Panel on Infant Formula and Adult Nutritionals; thus, the method was determined to be appropriate for First Action status.
在2011年6月29日举行的“标准制定与国际协调：AOAC国际年中会议”上，一个专家评审小组一致同意将“采用免疫亲和萃取液相色谱/紫外检测法测定婴儿配方食品和成人营养食品中的维生素B12”这一方法列为AOAC官方首次行动状态。该方法适用于测定维生素B12，其中包括添加的氰钴胺素和天然形式，适用于强化和非强化产品。维生素B12在氰化钠存在下于醋酸钠缓冲液中从样品中提取（100摄氏度，30分钟）。经免疫亲和柱纯化和浓缩后，用紫外检测（361纳米）的液相色谱法测定维生素B12。对一系列产品进行了单实验室验证研究，包括牛奶基和大豆基婴儿配方食品、谷物、可可饮料、保健品和多种维生素预混料。该方法在很宽的浓度范围内显示出线性响应，回收率为100.8±7.5%（平均值±标准差），重复性相对标准偏差（RSDr）为2.1%，中间精密度（RSD(iR)）为4.3%。检测限和定量限分别为0.10和0.30微克/100克，与参考微生物分析法的相关性良好（R2 = 0.9442）。研究结果发表在《美国分析化学家协会国际杂志》91, 786-793 (2008)上。该方法的性能特征符合婴儿配方食品和成人营养食品利益相关方小组规定的标准方法性能要求；因此，该方法被确定适合列为首次行动状态。
