Schimpf Karen, Spiegel Renee, Thompson Linda, Dowell Dawn
Abbott Laboratories, 3300 Stelzer Rd, Columbus, OH 43219, USA.
J AOAC Int. 2012 Mar-Apr;95(2):313-8. doi: 10.5740/jaoacint.cs2011_10.
During the "Standards Development and International Harmonization: AOAC INTERNATIONAL Mid-Year Meeting" held on June 29, 2011, an Expert Review Panel (ERP) reviewed the method "Determination of Vitamin B12 in Infant Formula and Adult Nutritionals by HPLC." Under the new pathway to Official Methods, the ERP adopted the method as Official First Action. The method is applicable to the determination of vitamin B12 in infant formula and adult nutritionals. Data showed an average overall intermediate precision of 6.64% RSD, an estimated quantitation limit of 0.8 microg/kg, and a detection limit of 0.2 microg/kg in prepared samples. The standard range of the method is 2 to 200 microg/L, which corresponds to an analytical range of 0.8 to 500 microg/kg.
在2011年6月29日举行的“标准制定与国际协调：AOAC国际年中会议”上，一个专家评审小组（ERP）对“用高效液相色谱法测定婴儿配方食品和成人营养食品中的维生素B12”这一方法进行了评审。根据官方方法的新途径，ERP采用该方法作为官方首次行动方法。该方法适用于测定婴儿配方食品和成人营养食品中的维生素B12。数据显示，在制备好的样品中，平均总体中间精密度为相对标准偏差（RSD）6.64%，估计定量限为0.8微克/千克，检测限为0.2微克/千克。该方法的标准范围为2至200微克/升，相当于分析范围为0.8至500微克/千克。
